Wide field of view common-path lateral-shearing digital holographic interference microscope

Vora, Priyanka; Trivedi, Vismay; Mahajan, Swapnil; Patel, Nimit; Joglekar, Mugdha; Chhaniwal, Vani K.; Moradi, Ali‐Reza; Javidi, Bahram; Anand, Arun

doi:10.1117/1.jbo.22.12.126001

Cited by 16 publications

(7 citation statements)

References 38 publications

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“…A reference hologram is recorded for calculating the background phase and finally subtracted to recover the final phase. The phase difference was calculated using the formulae [22];…”

Section: Resultsmentioning

confidence: 99%

Speckle-free common-path quantitative phase imaging with high temporal phase stability using a partially spatially coherent multi-spectral light source

et al. 2019

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Quantitative phase imaging (QPI) has been an important technique for the determination of various biophysical parameters, such as the refractive index and morphology of biological cells and tissues. The technique has been implemented using off-axis and common path digital holographic microscopy (DHM). Off-axis DHM, though being ideal for QPI, offers lower temporal phase stability compared to common path techniques. Common path DHM offers a higher temporal phase stability but, being a coherent imaging technique, suffers from speckle artefacts and spurious interference fringes due to the high coherence of the light source. Both artefacts not only degrade the image quality, but also affect the phase measurement results. In this paper, we report the implementation of common path QPI set-up using a partially spatially coherent multi-spectral light source. The spatial coherence of the red and green color lasers is reduced by using a rotating diffuser. Also, a speckle free QPI was implemented using common path Fresnel biprism interferometer. A Fresnel biprism is used in the self-referencing mode, thus offering the advantage of no optical power loss in addition to high temporal stability and the least speckle artefacts. Furthermore, it is very easy to implement, as the system completely replaces the need for spatial filtering at the source end as well as for the reference beam generation. The experiments have been carried out on industrial and biological samples to prove the effect.

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“…A reference hologram is recorded for calculating the background phase and finally subtracted to recover the final phase. The phase difference was calculated using the formulae [22];…”

Section: Resultsmentioning

confidence: 99%

Speckle-free common-path quantitative phase imaging with high temporal phase stability using a partially spatially coherent multi-spectral light source

et al. 2019

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“…The enhancement of resolution under tilted illumination can also be explained by equation ( 3) [20]. The two spectra belonging to the first and the second wave-fronts are both band-pass filtered by the 'rect' function, however, thanks to the oblique light the band-pass filter selects a different frequency band of the object.…”

Section: Theoretical Considerationsmentioning

confidence: 99%

“…This imposes serious restrictions on the sample type and the field of view. To overcome this shortcoming, it has been proposed to apply low-pass filtering on one of the wavefronts in order to remove the object information from it [16,19,20]. However, this solution, although being effective, might be challenging regarding specific pinhole fabrication and loss of intensity on the filtered wave-front (thus lower fringe contrast).…”

Section: Introductionmentioning

confidence: 99%

Oblique illumination lateral shearing digital holographic microscopy

Ganjkhani¹,

Calabuig²,

Pedrini³

et al. 2020

J. Opt.

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In this paper we present oblique illumination lateral shearing digital holographic microscopy (OILS-DHM) to remove the overlapping information of one of the two wave-fronts forming the holograms and enhance the lateral resolution. Lateral shearing DHM as a self-referencing scheme makes use of the interference of the object beam with itself to form the hologram, and thus is a compact and simple technique and is immune to environmental vibrations. Nonetheless, like all self-referencing techniques it suffers from restrictions on the sample and the field of view. We show that by applying oblique illumination in lateral shearing DHM, it is possible to remove the overlapping information of one of the interfering wave-fronts and thus lift the restriction on the sample. Furthermore, due to the intrinsic characteristic of oblique illumination the lateral resolution of the holographic imaging system is enhanced. The technique is validated through conducting experiments on 1D and 2D static objects. The presented methodology can be extended to other off-axis DHM schemes, and may be considered as a highly stable benchtop 3D imaging microscope for variety of samples.

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“…These complications can be overcome by employing a common-path configuration. [35][36][37][38][39][40][41][42][43] In common-path DHM systems, the object and reference beams follow approximately the same path and, therefore, make the systems less prone to environmental vibrations/perturbations. However, in such systems, both beams carry identical information and have enormous chances of overlapping at the image sensor.…”

Section: Introductionmentioning

confidence: 99%

Common-path multimodal three-dimensional fluorescence and phase imaging system

et al. 2020

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A stable multimodal system is developed by combining two common-path digital holographic microscopes (DHMs): coherent and incoherent, for simultaneous recording and retrieval of three-dimensional (3-D) phase and 3-D fluorescence imaging (FI), respectively, of a biological specimen. The 3-D FI is realized by a single-shot common-path off-axis fluorescent DHM developed recently by our group. In addition, we accomplish, the phase imaging by another single-shot, highly stable common-path off-axis DHM based on a beam splitter. In this DHM configuration, a beam splitter is used to divide the incoming object beam into two beams. One beam serves as the object beam carrying the useful information of the object under study, whereas another beam is spatially filtered at its Fourier plane by using a pinhole and it serves as a reference beam. This DHM setup, owing to a common-path geometry, is less vibration-sensitive and compact, having a similar field of view but with high temporal phase stability in comparison to a two-beam Mach-Zehnder-type DHM. The performance of the proposed common-path DHM and the multimodal system is verified by conducting various experiments on fluorescent microspheres and fluorescent protein-labeled living cells of the moss Physcomitrella patens. Moreover, the potential capability of the proposed multimodal system for 3-D live fluorescence and phase imaging of the fluorescent beads is also demonstrated. The obtained experimental results corroborate the feasibility of the proposed multimodal system and indicate its potential applications for the analysis of functional and structural behaviors of a biological specimen and enhancement of the understanding of physiological mechanisms and various biological diseases. © The Authors. Published by SPIE under a Creative Commons Attribution 4.0 Unported License. Distribution or reproduction of this work in whole or in part requires full attribution of the original publication, including its DOI.

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Wide field of view common-path lateral-shearing digital holographic interference microscope

Cited by 16 publications

References 38 publications

Speckle-free common-path quantitative phase imaging with high temporal phase stability using a partially spatially coherent multi-spectral light source

Speckle-free common-path quantitative phase imaging with high temporal phase stability using a partially spatially coherent multi-spectral light source

Oblique illumination lateral shearing digital holographic microscopy

Common-path multimodal three-dimensional fluorescence and phase imaging system

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