Wine pomace seasoning attenuates hyperglycaemia-induced endothelial dysfunction and oxidative damage in endothelial cells

Pino-García, Raquel Del; Gerardi, Gisela; Rivero-Pérez, María D.; García‐Lomillo, Javier

doi:10.1016/j.jff.2016.02.001

Cited by 20 publications

(15 citation statements)

References 80 publications

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“…In particular, NO content in the I3R treatment at 40 μM was the highest at 2.37 ± 0.01 μmol/L, which presented no significant difference from that in the control group ( p > 0.05), followed by C3G and then A7G. Several previous studies have found that maintaining NO bioavailability in vascular endothelial cells by dietary polyphenols is beneficial for improving endothelial dysfunction caused by diabetes [ 7 , 22 , 24 ]. Gerardi et al reported that the gastrointestinal digestive products of wine lees contain a high content of flavonols that can regulate endothelial permeability by maintaining NO production [ 7 ].…”

Section: Resultsmentioning

confidence: 82%

“…Moreover, resveratrol can stimulate AMP-activated protein kinase to activate eNOS to produce NO, eventually improving vascular endothelial dysfunction in type 2 diabetes [ 22 ]. Similarly, Garcia et al found that wine pomace seasoning rich in a variety of flavonoids significantly increased NO bioavailability in EA.hy926 cells in a high-glucose environment [ 24 ].…”

Section: Resultsmentioning

confidence: 99%

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Dietary Flavonoids Alleviate Inflammation and Vascular Endothelial Barrier Dysfunction Induced by Advanced Glycation End Products In Vitro

Jia

Sun

et al. 2022

Nutrients

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The aim of this study was to compare the protective effects of three dietary flavonoids (apigenin-7-O-glucoside (A7G), isorhamnetin-3-O-rutinoside (I3R), and cyanidin-3-O-glucoside (C3G)) on advanced glycation end products (AGEs)-induced inflammation and vascular endothelial dysfunction. Furthermore, the potential mechanisms of varied effects of those three dietary flavonoids were analyzed by molecular docking analysis. Results showed that C3G (40 μM) achieved the best inhibition on inflammatory cytokines (TNF-α, IL-1β, and IL-6) in AGEs-induced RAW264.7 cells, followed by I3R, and A7G was the weakest. The molecular docking results also showed that C3G exhibited the closest binding with the receptor for AGE. However, I3R (40 μM) demonstrated the best effect in improving endothelial dysfunction in AGEs-induced EA.hy926 cells, followed by C3G, and A7G was the weakest, as evidenced by the molecular docking results of flavonoids with profilin-1. This work may provide knowledge and helpful suggestions regarding the benefits of dietary flavonoids in diabetic vascular complications.

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Section: Resultsmentioning

confidence: 82%

Section: Resultsmentioning

confidence: 99%

Dietary Flavonoids Alleviate Inflammation and Vascular Endothelial Barrier Dysfunction Induced by Advanced Glycation End Products In Vitro

Jia

Sun

et al. 2022

Nutrients

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“…Plasma reduced glutathione (GSH) and oxidized glutathione (GSSG) levels were determined using the reaction between the sulfhydryl group of the GSH and 5,5 -dithio-bis-2-nitrobenzoic acid (DTNB, Ellman's reagent) [23]. The GSTNB (mixture between GSH and TNB) is reduced by glutathione reductase to recycle GSH and produce more TNB.…”

Section: Plasma Glutathione Reduced/oxidized (Gsh/gssg) Ratio Analysismentioning

confidence: 99%

Effects of Hypothermia and Allopurinol on Oxidative Status in a Rat Model of Hypoxic Ischemic Encephalopathy

et al. 2021

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Hypoxic ischemic encephalopathy (HIE) is one of the main causes of morbidity and mortality during the neonatal period, despite treatment with hypothermia. There is evidence that oxidative damage plays an important role in the pathophysiology of hypoxic-ischemic (HI) brain injury. Our aim was to investigate whether postnatal allopurinol administration in combination with hypothermia would reduce oxidative stress (OS) biomarkers in an animal model of HIE. Postnatal 10-day rat pups underwent unilateral HI of moderate severity. Pups were randomized into: Sham operated, hypoxic-ischemic (HI), HI + allopurinol (HIA), HI + hypothermia (HIH), and HI + hypothermia + allopurinol (HIHA). Biomarkers of OS and antioxidants were evaluated: GSH/GSSG ratio and carbonyl groups were tested in plasma. Total antioxidant capacity (TAC) was analyzed in plasma and cerebrospinal fluid, and 8-iso-prostaglandin F2α was measured in brain tissue. Plasma 2,2′–azinobis-(3-ethyl-benzothiazoline-6-sulfonic acid) (ABTS) levels were preserved in those groups that received allopurinol and dual therapy. In cerebrospinal fluid, only the HIA group presented normal ferric reducing ability of plasma (FRAP) levels. Protein oxidation and lipid peroxidation were significantly reduced in all groups treated with hypothermia and allopurinol, thus enhancing neuroprotection in HIE.

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“…The nutrient composition, total ( poly)phenol contents and antioxidant capacities of the supplement are shown in Table 1. Methods used for the compositional analysis of RWPS, 23,24 a more detailed determination of the individual phenolic composition of RWPS, 21 and the bioavailability of phenolic compounds after acute administration of 300 mg kg −1 RWPS in healthy rats 25 have been previously published.…”

Section: Animals and Experimental Proceduresmentioning

confidence: 99%

“…18,19 Wine pomace is also a great source of dietary fiber, thus being a phenolic-rich dietary fiber matrix with numerous potential benefits on human health. 20 In the present study, a natural seasoning obtained from seedless red wine pomace (RWPS), whose potential vascular protection under hyperglycemic conditions has been suggested from previous studies in human umbilical vein endothelial cells (HUVECs), 21 was given as a dietary supplement to a rat model of Type 1 DM. The main purpose was to: firstly, evaluate the potential hypoglycemic and protective effects of RWPS against oxidative stress and associated endothelial dysfunction; and, secondly, provide mechanistic insight into the gene expression modulation of key genes involved in vascular homeostasis.…”

Section: Introductionmentioning

confidence: 97%

Attenuation of oxidative stress in Type 1 diabetic rats supplemented with a seasoning obtained from winemaking by-products and its effect on endothelial function

et al. 2016

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Type 1 diabetes mellitus (DM) is characterized by hyperglycemia resulting from insulin deficiency. This is usually accompanied by a pro-oxidative environment, dyslipidemia and endothelial dysfunction, thus leading to several micro- and macro-vascular complications. This study investigated the potential benefits of a seasoning obtained from seedless red wine pomace (RWPS) in protecting against oxidative damage and preserving endothelial function in Type 1 DM, and the underlying mechanisms involved at the level of gene expression. The diet of streptozotocin (45 mg kg)-induced diabetic (DB) and control (CN) male Wistar rats (n = 5 rats per group) was supplemented with RWPS (300 mg per kg per day) or vehicle for 4 weeks. Characteristic indicators of DM such as increased food and water intakes and weight loss were significantly ameliorated in DB + RWPS rats, with a notable normalization in their fasting glycemic control and cholesterol profile. Plasma total antioxidant capacity (TAC) was substantially increased, and biomarkers of oxidative damage to lipids (F-isoprostanes, 24.9%; malondialdehyde, 28.4%) and proteins (carbonyl groups, 5.91%) were significantly decreased. Nitric oxide availability tended to improve in plasma of DB + RWPS compared with DB rats. Insulin levels were increased (1.51-fold) and aortic tissue antioxidant enzymes such as mitochondrial superoxide dismutase (SOD2, 1.93-fold) were up-regulated. Other important genes for endothelial function, including endothelial β-nicotinamide adenine dinucleotide phosphate oxidase (NOX4), endothelial and inducible nitric oxide synthases (eNOS, iNOS), and angiotensin-converting enzyme-I (ACE), were non-significantly modulated, although certain potentially positive trends were observed. These results indicate that RWPS supplementation might be a useful nutritional approach to manage Type 1 DM and ameliorate its vascular complications.

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Wine pomace seasoning attenuates hyperglycaemia-induced endothelial dysfunction and oxidative damage in endothelial cells

Cited by 20 publications

References 80 publications

Dietary Flavonoids Alleviate Inflammation and Vascular Endothelial Barrier Dysfunction Induced by Advanced Glycation End Products In Vitro

Dietary Flavonoids Alleviate Inflammation and Vascular Endothelial Barrier Dysfunction Induced by Advanced Glycation End Products In Vitro

Effects of Hypothermia and Allopurinol on Oxidative Status in a Rat Model of Hypoxic Ischemic Encephalopathy

Attenuation of oxidative stress in Type 1 diabetic rats supplemented with a seasoning obtained from winemaking by-products and its effect on endothelial function

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