Wolbachia Strain wAlbB Enhances Infection by the Rodent Malaria Parasite Plasmodium berghei in Anopheles gambiae Mosquitoes

Hughes, Grant L.; Vega-Rodríguez, Joel; Xue, Ping; Rasgon, Jason L.

doi:10.1128/aem.06751-11

Cited by 114 publications

(127 citation statements)

References 44 publications

(73 reference statements)

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“…Eliminating Asaia may facilitate the development of Wolbachia infections in these other vectors. The creation of Anopheles mosquito lines transinfected with a variety of Wolbachia strains will be worthwhile, given that some Anopheles-Wolbachia strain combinations may not be suitable for malaria control due to their propensity to increase certain Plasmodium parasites (36,56,57).…”

Section: Discussionmentioning

confidence: 99%

“…The mortality dynamics of mosquitoes pre-and postblood meal were conducted as previously described (35,36). wAlbB was injected into mosquitoes reared on conventional sugar, reared continuously on antibiotics, or reared continuously on antibiotics but supplemented with Asaia R as described above.…”

Section: Methodsmentioning

confidence: 99%

“…With few exceptions, Anophelines (which transmit the Plasmodium parasites that cause human malaria) are naturally uninfected with Wolbachia (28-31), suggesting the potential presence of innate barriers to infection in this genus. However, in vitro and in vivo studies indicate that Wolbachia are capable of infecting cultured Anopheles cells (32,33), ex vivo cultured tissues (34), in vivo somatic tissue (35)(36)(37), and can stably infect the mosquito germ line (38). We investigated the ability of the native microbial community to influence vertical transmission of Wolbachia in Anopheles mosquitoes.…”

Section: Significancementioning

confidence: 99%

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Native microbiome impedes vertical transmission of Wolbachia in Anopheles mosquitoes

Hughes¹,

Dodson²,

Johnson

et al. 2014

Proc. Natl. Acad. Sci. U.S.A.

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230

222

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Significance Factors influencing Wolbachia transfer into new species remain poorly understood. This is important as Wolbachia can influence speciation and is being developed as a novel arthropod-borne disease control approach. We show the native microbiota of Anopheles impede vertical transmission of Wolbachia . Antibiotic microbiome perturbation enables Wolbachia transmission in two Anopheles species. Mosquitoes with altered microbiomes do not exhibit blood meal-induced mortality associated with Wolbachia infection, suggesting that mosquitoes are killed by interactions between Wolbachia and other bacteria present in the mosquito. We identified Asaia as the bacterium responsible for inhibiting Wolbachia transmission, and partially responsible for blood meal-induced mortality. These results suggest that microbial interactions profoundly affect the host, and that microbiome incompatibility may influence distribution of Wolbachia in arthropods.

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Section: Discussionmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%

Section: Significancementioning

confidence: 99%

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Native microbiome impedes vertical transmission of Wolbachia in Anopheles mosquitoes

Hughes¹,

Dodson²,

Johnson

et al. 2014

Proc. Natl. Acad. Sci. U.S.A.

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230

222

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“…Though originally developed for IIT and life-shortening strategies, it was observed that infection with certain strains of Wolbachia dramatically reduced susceptibility to a range of pathogens, [99][100][101] though potentially increasing susceptibility to others. 102 Wolbachia are capable of spreading through insect populations as a heritable modification by manipulating the host's reproductive biology 84,103 -in other words, Wolbachia has the properties of a gene drive system. This raised the possibility that certain strains of Wolbachia might provide a complete gene-drive-plus-refractory-gene package.…”

Section: Gene Drive Systemsmentioning

confidence: 99%

Genetic control ofAedesmosquitoes

Alphey

McKemey

Nimmo

et al. 2013

Pathogens and Global Health

134

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Aedes mosquitoes include important vector species such as Aedes aegypti, the major vector of dengue. Genetic control methods are being developed for several of these species, stimulated by an urgent need owing to the poor effectiveness of current methods combined with an increase in chemical pesticide resistance. In this review we discuss the various genetic strategies that have been proposed, their present status, and future prospects. We focus particularly on those methods that are already being tested in the field, including RIDL and Wolbachia-based approaches.

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“…Best known for its ability to invade invertebrate populations via modification of host reproductive systems (4), some Wolbachia-infected insects are protected from viruses and other pathogens (5)(6)(7)(8)(9)(10)(11)(12), while others have enhanced infection (13)(14)(15)(16)(17). Due to this ability to disrupt virus infection, there is an increased interest in employing Wolbachia as a means of biological control of arthropod-transmitted infectious diseases, such as dengue virus (18).…”

mentioning

confidence: 99%

Oxidative Stress Correlates with Wolbachia-Mediated Antiviral Protection in Wolbachia-Drosophila Associations

Wong

Brownlie

Johnson

2015

Appl Environ Microbiol

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bWolbachia mediates antiviral protection in insect hosts and is being developed as a potential biocontrol agent to reduce the spread of insect-vectored viruses. Definition of the molecular mechanism that generates protection is important for understanding the tripartite interaction between host insect, Wolbachia, and virus. Elevated oxidative stress was previously reported for a mosquito line experimentally infected with Wolbachia, suggesting that oxidative stress is important for Wolbachia-mediated antiviral protection. However, Wolbachia experimentally introduced into mosquitoes impacts a range of host fitness traits, some of which are unrelated to antiviral protection. To explore whether elevated oxidative stress is associated with antiviral protection in Wolbachia-infected insects, we analyzed oxidative stress of five Wolbachia-infected Drosophila lines. In flies infected with protective Wolbachia strains, hydrogen peroxide concentrations were 1.25-to 2-fold higher than those in paired fly lines cured of Wolbachia infection. In contrast, there was no difference in the hydrogen peroxide concentrations in flies infected with nonprotective Wolbachia strains compared to flies cured of Wolbachia infection. Using a Drosophila mutant that produces increased levels of hydrogen peroxide, we investigated whether flies with high levels of endogenous reactive oxygen species had altered responses to virus infection and found that flies with high levels of endogenous hydrogen peroxide were less susceptible to virusinduced mortality. Taken together, these results suggest that elevated oxidative stress correlates with Wolbachia-mediated antiviral protection in natural Drosophila hosts.T he maternally inherited endosymbiont Wolbachia pipientis is an alphaproteobacterium predicted to infect at least 40% of insect species (1-3). Best known for its ability to invade invertebrate populations via modification of host reproductive systems (4), some Wolbachia-infected insects are protected from viruses and other pathogens (5-12), while others have enhanced infection (13-17). Due to this ability to disrupt virus infection, there is an increased interest in employing Wolbachia as a means of biological control of arthropod-transmitted infectious diseases, such as dengue virus (18). Despite this interest, the mechanisms of Wolbachia antiviral protection remain to be fully elucidated.Several studies have provided insight into Wolbachia-mediated antiviral protection. Wolbachia can mediate broad protection against a range of different RNA viruses in both Drosophila species and mosquitoes (5-12). Wolbachia-infected Drosophila flies are concomitantly protected against two diverse viruses, Drosophila C virus (DCV; Dicistroviridae) and Flock House virus (FHV; Nodaviridae), and the protection is strongly genetically correlated (6,11,12,19). These findings suggest that Wolbachia mediates antiviral protection through a mechanism with broad specificity. Interestingly, Wolbachia infection often interferes with virus accumulation, but examples have...

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Wolbachia Strain wAlbB Enhances Infection by the Rodent Malaria Parasite Plasmodium berghei in Anopheles gambiae Mosquitoes

Cited by 114 publications

References 44 publications

Native microbiome impedes vertical transmission of Wolbachia in Anopheles mosquitoes

Native microbiome impedes vertical transmission of Wolbachia in Anopheles mosquitoes

Genetic control ofAedesmosquitoes

Oxidative Stress Correlates with Wolbachia-Mediated Antiviral Protection in Wolbachia-Drosophila Associations

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