Wound healing and antibacterial properties of methanolic extract of Pupalia lappacea Juss in rats

Abstract

BackgroundWound healing is a natural process that enables tissue repair after an injury. To shorten its duration and minimize associated complications, wounds are treated with medications. Currently there is a growing interest in the use of alternative wound dressing agents such as plant extracts. One plant used traditionally in wound treatment is Pupalia lappacea. In view of its use in wound care, we investigated the wound healing activities of 80% methanolic leave extract of Pupalia lappacea using excision, incision and dead space wound models. Also its effects on three common wound contaminants were investigated.MethodsExcision wounds were created, contaminated with microbes and treated with ointments (10% and 20% w/w) prepared from Pupalia lappacea. Incision and dead space wounds were also created in rats which were subsequently dosed orally with the extract. The wound healing activities of Pupalia lappacea ointment on excision wound was assessed by rates of wound contraction and epithelialization as well as its antibacterial effects. The effects of Pupalia lappacea on incision and dead-space wounds were determined by the wound breaking strengths and weights of the granuloma tissues formed, respectively.ResultsPupalia. lappacea ointments significantly (p < 0.05) accelerated wound healing with 20% ointment having the highest percentage wound contraction and rate of epithelialization. At 4, 7 and 14 days post treatment, mean total viable bacterial count of excision wounds of the extract treated groups were significantly (p < 0.05) lower compared against the control. Wound breaking strengths and weights of granuloma tissues formed in the extract treated groups were significantly (p < 0.05) higher than those of the control group. The minimum inhibitory concentration values obtained for the Pupalia lappacea extract against Pseudomonas aeruginosa, Staphylococcus aureus and Bacillus subtilis were 9 mg/ml, 4 mg/ml and 3 mg/ml, respectively, while the corresponding minimum bactericidal concentrations were 10 mg/ml, 8 mg/ml and 7 mg/ml.ConclusionThe results obtained showed that Pupalia. lappacea has good wound healing and antibacterial activities. These findings validate the use of this plant in traditional medicine for treatment of wounds.

UHPLC-DAD characterization of bioactive secondary metabolites from Ocimum americanum and Pupalia lappacea extracts: Antioxidant activity and antihypertensive effects on L-NAME-induced hypertensive rats

“…However, differences were observed with coumarins (Dibala et al, 2016), alkaloids (Enemali and Udedi, 2018) and tannins (Elya et al, 2015). Regarding P. lappacea, a similitude was also observed with previous study (Udegbunam et al, 2014;Hoekou et al, 2012). Contrary to our results, alkaloids and tannins have been detected in ethanolic extract.…”

Medicinal Plants and Natural Products with Demonstrated Wound Healing Properties

“…The ethanol and chloroform extracts also exhibited high rate of wound closure [25]. However, Udegbunam et al [26] also reported that higher concentrations (10% and 20% (w/v) ointment of methanol leaf extract of P. lappacea) significantly (p < 0.05) accelerated wound healing, thereby the 20% ointment having the highest percentage wound contraction and rate of epithelialization. The extract also exhibited antimicrobial activity with MIC of 3.0-9.0 mg/mL and MBC of 7-10 mg/ mL against Pseudomonas aeruginosa, Staphylococcus aureus and Bacillus subtilis.…”

A review on medicinal plant extracts and their active ingredients against methicillin-resistant and methicillin-sensitive Staphylococcus aureus

“…The petroleum ether extract of the plant has an excellent antibacterial activity against MRSA with an IC50 value of 7.8 μg/mL (82). The extract of Laurus nobilis leaves has also shown a potent anti-MRSA activity (ZOI = 25 mm) and an antiquorum sensing activity (>17 mm) (46). Ethyl acetate fraction from Cinnamomum iners standardized leave has a strong antibacterial activity, especially against MRSA and E. coli with the MIC values of 100 and 200 μg/mL, respectively.…”