Wound-healing effect of micronized sacchachitin (mSC) nanogel on corneal epithelium

Abstract

Abstract:The extraction residue of the Ganoderma fruiting body, named sacchachitin, has been demonstrated to have the potential to enhance cutaneous wound healing by inducing cell proliferation. In this study, a nanogel formed from micronized sacchachitin (mSC) was investigated for the potential treatment of superficial chemical corneal burns. Reportedly, mSC has been produced successfully and its chemical properties confirmed, and physical and rheological properties characterized. An in vitro cell proliferation study has revealed that at the concentrations of 200, 300, and 400 µg/mL, mSC nanogel significantly increased Statens Seruminstitut rabbit corneal (SIRC) cell proliferation after 24 hours of incubation. In cell migration assay, migration of SIRC cell to wound closure was observed after 24 hours of incubation with the addition of 200 µg/mL mSC of nanogel. In an animal study, acceleration of corneal wound healing was probably due to the inhibition of proteolysis. In conclusion, the findings of this study substantiate the potential application of sacchachitin in the form of mSC nanogel for the treatment of superficial corneal injuries.

Micronized sacchachitin promotes satellite cell proliferation through TAK1-JNK-AP-1 signaling pathway predominantly by TLR2 activation

“…This indicated that those short fibers of mSC was so small in both length and diameter that they could be hydrated to become nanogel when mSC was dispersed in aqueous medium. Rheological characteristics of m SC nanogel have also been reported by Chen et al [ 29 ]. Please also see Additional file 1 for details.…”

“…Physical characteristics of m SC have been reported by Chen et al [ 29 ]. It was summarized as the following.…”

“…The concentration of mSC could possibly vary its effect [ 29 ]; therefore, we determined its optimal concentration, which could effectively promote SC proliferation. The primary cultured myogenic cells, which were isolated from the TA muscle, were cocultured in growth media that contained different concentrations of mSC, such as 2 mg/mL, 200 µg/mL, and 20 µg/mL.…”

“…In this study, the SCs were isolated from the tibialis anterior (TA) muscle of a healthy rat. These isolated SCs were then cultured in growth media containing different concentrations of micronized sacchachitin (mSC), a novel chitin-polysaccharide extracted from the residue of the Ganoderma fruiting body, which can induce the proliferation of the corneal epithelium according to our previous reports [ 2 , 28 , 29 ]. In this study, we successfully identified the optimal concentration of mSC for promoting the proliferation of SCs through co-immunostaining of Pax7 and bromodeoxyuridine (BrdU) assay.…”

Borrowing From Nature: Biopolymers and Biocomposites as Smart Wound Care Materials

“…In a following study (Su et al, 2005 ), the Sacchachitin membranes appeared to boost keratinocytes proliferation and prevent metalloproteinase-related ECM degradation, contributing to accelerate the healing process of a chronic wound in vivo model. Furthermore, a micronized Sacchachitin nanogel has been investigated to treat superficial chemical corneal burns in vivo (Chen et al, 2012 ), while the anti-oxidant and immuno-modulating effects of extracts from mycelia of some medicinal fungi have been investigated for skin aging (Kim et al, 2014 ), dermatitis (Hwang et al, 2012 ), and UV-protection (Nanbu et al, 2011 ; Bae et al, 2012 ), suggesting a promising mycelia biological value yet unexplored.…”