Wound-induced assembly and closure of an actomyosin purse string in Xenopus oocytes

Bement, William M.; Mandato, Craig A.; M, Kirsch

doi:10.1016/s0960-9822(99)80261-9

Cited by 200 publications

(239 citation statements)

References 36 publications

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“…(16) The actomyosin array segregates over time: while F-actin assembles throughout the wound array, myosin-2 becomes enriched on the interior circumference. (17) Subsequent studies showed that Rho GTPases direct the actomyosin response during wound healing and are likely responsible for the observed segregation of the actomyosin array. That is, wounding elicits simultaneous local activation of both Rho and Cdc42 about 20 seconds after wounding followed by segregation of active Rho and Cdc42 into complementary zones, with active Cdc42 circumscribing active Rho (Fig.…”

Section: Rho Gtpase Activity Zonesmentioning

confidence: 99%

“…During oocyte wound healing, for example, myosin-2 concentrates on the interior of the actomyosin array, while dynamic actin is concentrated around the outside of the array. (16,17) That this segregation results from the segregation of the Cdc42 and Rho activity zones is indicated both by the timing of segregation and the effects of experimentally broadening the Rho zone. (3) This raises a further question, namely, why segregate different components of the contractile array?…”

Section: Contributions Of the Zones To Contractile Arraysmentioning

confidence: 99%

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Rho GTPase activity zones and transient contractile arrays

2006

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SummaryThe Rho GTPases-Rho, Rac and Cdc42-act as molecular switches, cycling between an active GTP-bound state and an inactive GDP-bound state, to regulate the actin cytoskeleton. It has recently become apparent that the Rho GTPases can be activated in subcellular zones that appear semi-stable, yet are dynamically maintained. These Rho GTPase activity zones are associated with a variety of fundamental biological processes including symmetric and asymmetric cytokinesis and cellular wound repair. Here we review the basic features of Rho GTPase activity zones, suggest that these zones represent a fundamental signaling mechanism, and discuss the implications of zone properties from the perspective of both their function and how they are likely to be controlled.

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Section: Rho Gtpase Activity Zonesmentioning

confidence: 99%

Section: Contributions Of the Zones To Contractile Arraysmentioning

confidence: 99%

Rho GTPase activity zones and transient contractile arrays

2006

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“…Such an actin cable has been reported to play a role in the morphogenesis and wound healing of Drosophila embryos (Young et al 1993, Wood et al 2002, in injured singlecelled Xenopus oocytes (Bement et al 1999), in the healing of chick embryo epidermises (Martin & Lewis 1992) as well as in the repair of tissue culture wounds (Bement et al 1993, Altan & Fenteany 2004. It has been suggested that the actin cable functions as a "purse string" contracting the wound edges together (reviewed by Kiehart 1999).…”

Section: Imaginal Disc Wound Healingmentioning

confidence: 99%

Role of Jun N-terminal Kinase (JNK) signaling in the wound healing and regeneration of a Drosophila melanogaster wing imaginal disc

Mattila¹,

Omelyanchuk²,

Kyttala³

et al. 2005

Int. J. Dev. Biol.

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When a fragment of a Drosophila imaginal disc is cultured in growth permissive conditions, it either regenerates the missing structures or duplicates the pattern present in the fragment. This kind of pattern regulation is known to be epimorphic, i.e. the new pattern is generated by proliferation in a specialized tissue called the blastema. Pattern regulation is accompanied by the healing of the cut surfaces restoring the continuous epithelia. Wound healing has been considered to be the inductive signal to commence regenerative cell divisions. Although the general outlines of the proliferation dynamics in a regenerating imaginal disc blastema have been well studied, little is known about the mechanisms driving cells into the regenerative cell cycles. In this study, we have investigated the role of Jun N-terminal Kinase (JNK) signaling in the wound healing and regeneration of a Drosophila wing imaginal disc. By utilizing in vivo and in vitro culturing of incised and fragmented discs, we have been able to visualize the dynamics in cellular architecture and gene expression involved in the healing and regeneration process. Our results directly show that homotypic wound healing is not a prerequisite for regenerative cell divisions. We also show that JNK signaling participates in imaginal disc wound healing and is regulated by the physical dynamics of the process, as well as in recruiting cells into the regenerative cell cycles. A model describing the determination of blastema size is discussed.

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“…Membrane resealing is a conserved process by which cells are able to survive mechanical disruption of the plasma membrane (14)(15)(16)(17)(18)(19). Dysferlin-null skeletal and cardiac muscle show enhanced uptake of membrane impermeable dye following laser-induced wounding, suggesting that dysferlin may play a role in membrane resealing (3,20).…”

Section: Introductionmentioning

confidence: 99%

Membrane damage-induced vesicle–vesicle fusion of dysferlin-containing vesicles in muscle cells requires microtubules and kinesin

McDade

Michele

2013

Human Molecular Genetics

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Mutations in the dysferlin gene resulting in dysferlin-deficiency lead to limb-girdle muscular dystrophy 2B and Myoshi myopathy in humans. Dysferlin has been proposed as a critical regulator of vesicle-mediated membrane resealing in muscle fibers, and localizes to muscle fiber wounds following sarcolemma damage. Studies in fibroblasts and urchin eggs suggest that trafficking and fusion of intracellular vesicles with the plasma membrane during resealing requires the intracellular cytoskeleton. However, the contribution of dysferlin-containing vesicles to resealing in muscle and the role of the cytoskeleton in regulating dysferlin-containing vesicle biology is unclear. Here, we use live-cell imaging to examine the behavior of dysferlin-containing vesicles following cellular wounding in muscle cells and examine the role of microtubules and kinesin in dysferlin-containing vesicle behavior following wounding. Our data indicate that dysferlin-containing vesicles move along microtubules via the kinesin motor KIF5B in muscle cells. Membrane wounding induces dysferlin-containing vesicle-vesicle fusion and the formation of extremely large cytoplasmic vesicles, and this response depends on both microtubules and functional KIF5B. In non-muscle cell types, lysosomes are critical mediators of membrane resealing, and our data indicate that dysferlincontaining vesicles are capable of fusing with lysosomes following wounding which may contribute to formation of large wound sealing vesicles in muscle cells. Overall, our data provide mechanistic evidence that microtubulebased transport of dysferlin-containing vesicles may be critical for resealing, and highlight a critical role for dysferlin-containing vesicle-vesicle and vesicle-organelle fusion in response to wounding in muscle cells.

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Wound-induced assembly and closure of an actomyosin purse string in Xenopus oocytes

Cited by 200 publications

References 36 publications

Rho GTPase activity zones and transient contractile arrays

Rho GTPase activity zones and transient contractile arrays

Role of Jun N-terminal Kinase (JNK) signaling in the wound healing and regeneration of a Drosophila melanogaster wing imaginal disc

Membrane damage-induced vesicle–vesicle fusion of dysferlin-containing vesicles in muscle cells requires microtubules and kinesin

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