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Kadota, Masanori; Niimi, Yoshiji

doi:10.1023/a:1022378511659

Cited by 102 publications

(18 citation statements)

References 24 publications

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“…Considering this fact, it is highly recommended that the higher concentration of cytokinin component is necessary for the growth and differentiation of tissue culture [8]. It agrees with results obtained by Kadota and Niimi with pear ( Pyrus pyrifolia) [21] . They suggested that BAP displayed more noticeable effect and it was more suitable for shoot multiplication of pear.…”

Section: Discussionsupporting

confidence: 83%

“…Proper regeneration of adventitious shoots of carnation rely on composition of nutrient medium, plant grow regulators and types of explant [10], [15]. Cytokinin promotes cell division and cell expansion and growth is stimulated by adding a cytokinin to a proliferation medium [21]. Cytokinin induces the initiation and capability of axillary meristems which result in shoot formation.…”

Section: Discussionmentioning

confidence: 99%

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Micropropagation of carnation ( Dianthus caryophyllus L . ) in liquid medium by temporary immersion bioreactor in comparison with solid culture

Ahmadian

Babaei

Shokri

et al. 2017

Journal of Genetic Engineering and Biotechnology

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Developing scale-up system and automation of micropropagation in a bioreactor has been a possible way of cost reduction and intensive manual handling. We report a comparison between the results of experiments aimed at improving carnation micropropagation using new bioreactor according to Temporary Immersion Bioreactor (TIB) and solid culture. By applying different levels of BAP, at the concentration of 3 mg L−1, we observed 14.3 new shoots in TIB, but the number of new shoots on solid medium reached to 5.7 at the same treatment. Our results also showed that with 3 mg L−1 BAP in TIB, the initial fresh weight of plant material increased from 10 g to 450 g after 15 days. It is concluded that TIB showed more than 10 times shoot production of carnation. Shoot elongation and rooting induction was successfully stimulated in TIB by applying 1 mg L−1 IBA. Rooting of proliferated plantlets from TIB and solid culture were successfully happened, and led to highest number of roots (4.6 cm) and highest length of roots (6.87 cm) in TIB. More than 90% of plantlet was acclimatized to ex vitro. Our results suggested that mass production of carnation shoots in our simple TIB, with effective result, can be considered as a critical first step toward large scale production of carnation.

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Section: Discussionsupporting

confidence: 83%

Section: Discussionmentioning

confidence: 99%

Micropropagation of carnation ( Dianthus caryophyllus L . ) in liquid medium by temporary immersion bioreactor in comparison with solid culture

Ahmadian

Babaei

Shokri

et al. 2017

Journal of Genetic Engineering and Biotechnology

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“…Micropropagation is a reliable technique that plays a decisive role in rapid propagation of pear and researchers have claimed considerable success with this approach. The responses of pear cultivars to micropropagation are significantly variable, so that some cultivars have exhibited disorders like shoot tip necrosis [7], hyperhydricity [8–10], fascination [11] and hooked leaves [9], while some of them grow well [12].…”

Section: Introductionmentioning

confidence: 99%

“…That is why recent investigations have focused on optimization of the culture medium [10, 14, 15]. Several studies have been done on modifying MS medium by changing the level of plant growth regulators (PGRs) [8, 12, 16–18]. Wada et al [19] found that increasing mesos nutrients content of MS medium improves multiplication rate in several pear cultivars and alleviates some physiological disorders.…”

Section: Introductionmentioning

confidence: 99%

Combining gene expression programming and genetic algorithm as a powerful hybrid modeling approach for pear rootstocks tissue culture media formulation

et al. 2019

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BackgroundPredicting impact of plant tissue culture media components on explant proliferation is important especially in commercial scale for optimizing efficient culture media. Previous studies have focused on predicting the impact of media components on explant growth via conventional multi-layer perceptron neural networks (MLPNN) and Multiple Linear Regression (MLR) methods. So, there is an opportunity to find more efficient algorithms such as Radial Basis Function Neural Network (RBFNN) and Gene Expression Programming (GEP). Here, a novel algorithm, i.e. GEP which has not been previously applied in plant tissue culture researches was compared to RBFNN and MLR for the first time. Pear rootstocks (Pyrodwarf and OHF) were used as case studies on predicting the effect of minerals and some hormones in the culture medium on proliferation indices.ResultsGenerally, RBFNN and GEP showed extremely higher performance accuracy than the MLR. Moreover, GEP models as the most accurate models were optimized using genetic algorithm (GA). The improvement was mainly due to the RBFNN and GEP strong estimation capability and their superior tolerance to experimental noises or improbability.ConclusionsGEP as the most robust and accurate prospecting procedure to achieve the highest proliferation quality and quantity has also the benefit of being easy to use.

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“…Similarly, Rahman et al (2013) conducted tissue culture experiment and documented that that greater root length (3.69 cm) was depicted at 1.0 mg L -1 IBA for explants of banana. Kadota and Niimi (2003) also revealed that BAP had very important role in inducing and increasing number of shoots in banana genotypes. BAP is widely used and significantly affects the shoot induction in plants.…”

Section: Growth Traitsmentioning

confidence: 86%

Effects of 6-Benzylaminopurine and Indole-3-acetic Acid on Growth and Root Development of Banana Explants in Micropropagation

Khan¹,

Bashir²,

Erum³

et al. 2021

SJA

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The purpose of the experiment was to find out the impact of various combinations of growth regulators 6-Benzylaminopurine (BAP cytokinins family) and Indole-3-acetic acid (IAA auxin family) on growth and root induction of banana explants micro-propagated in vitro. Explants were obtained from young suckers of 8818-william, Pisang and Brazilian varieties. The explants were cultured in vitro containing MS media and different BAP and IAA combinations. The results showed banana varieties exhibited differences for the shoot and root development and also for the number of shoots and leaves. Wiallium-8818 hybrid gave more shoots (7.33) when the concentration of BAP and IAA was 2.5 and 1 mg L -1 , respectively. Alike, Wiallium-8818 hybrid produced the longest shoot (12.20 cm) with more leaves (6.23) at 1 mg L -1 of each BAP and IAA. But Pisang variety produced maximum fresh weight (15.45 g) with a greater number of roots at 5 + 1 mg L -1 of BAP + IAA, respectively.

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Cited by 102 publications

References 24 publications

Micropropagation of carnation ( Dianthus caryophyllus L . ) in liquid medium by temporary immersion bioreactor in comparison with solid culture

Micropropagation of carnation ( Dianthus caryophyllus L . ) in liquid medium by temporary immersion bioreactor in comparison with solid culture

Combining gene expression programming and genetic algorithm as a powerful hybrid modeling approach for pear rootstocks tissue culture media formulation

Effects of 6-Benzylaminopurine and Indole-3-acetic Acid on Growth and Root Development of Banana Explants in Micropropagation

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