XANTHINE OXYDASE INHIBITION OF KOMBUCHA TEA IN HYPERURICEMIA INDUCED WISTAR RAT: decrease of uric acid, malondialdehyde, and 8-hydroxy-2'-deoxyguanosine

Abstract

Background: Hyperuricemia is a condition of high level of uric acid in the body due to distortion of purine nucleoside metabolism through hipoxanthin, xanthin, and guanin of basic purine. Objective: to find a cure of hyperuricemia base on the utilization of kombucha tea. Methods: This is a true experimental study by applying posttest only control group design to determine whether kombucha tea inhibit xanthine oxidase in hyperuricemic induced rat reveales by decrease of uric acid, malondialdehyde (MDA), and 8-hydroxy-2'-deoxyguanosine (8-OHdG). In this study, hyperuricemia rat was achieved by intake of high purine diet. Rats were fed with a mixture of 4 g/kg BW of Gnetum gnemon with 50 mL/kg BW of chicken liver ad libitum for 9 days. Treatments in this research are combination of fermentation time of Kombucha tea and volume of this tea, i.e fermentation time 4, 8, and 12 days and the volume are 1 mL and 4 mL. Therefore, there would be seven groups of treatment including control group. ANOVA was then applied to determine the treatment effect with p < 0.05 was concidered significant. Results: This study indicates that kombucha tea has an ability to inhibit xanthine oxidase in hyperuricemic induced rat and decrease uric acid, MDA, and 8-OHdG. This ability was achieved with combination treatment of 12 days fermentation and 4 mL of kombucha intake. Xanthine oxidase, uric acid, MDA, and 8-OHdG levels by this treatment were obtained significantly lower compare to control group. Conclusion: This study proved that kombucha tea was potent to cure hyperuricemia of wistar rat via inhibition of xanthine oxidase produced.

Aktivitas Antihiperurisemia Ekstrak Etanol Daun Afrika (Vernonia amygdalina Delile.) pada Mencit Putih Galur Swiss Webster yang Diinduksi Melinjo dan Hati Ayam

“…Parameter yang diamati adalah kadar asam urat mencit. Hewan uji diinduksi hiperurisemia dengan pemberian suspensi biji melinjo dan jus hati ayam konstrasi 100% b/v secara oral selama 9 hari berturutturut kemudian diukur kadar asam uratnya (Sukrama, 2015). Setelah hewan uji mengalami hiperurisemia, maka diberikan sediaan pembanding, kontrol positif, dan sediaan uji ekstrak etanol daun Afrika selama 9 hari berturut-turut.…”

Beetroot Powder (Beta vulgaris L.) Decrease Oxidative Stress by Reducing of Malondialdehyde (MDA) Levels in Hyperuricemia

“…Increased uric acid production is also associated with reactive oxygen species (ROS) formation and results in increased lipid peroxidation. Lipid peroxidation produces MDA as a cell membrane damage markers (Sukrama, 2015).…”

Aktivitas antihiperurisemia teh asam daun tin (Ficus carica) terhadap tikus putih (Rattus norvegicus)

“…Fermentasi dapat meningkatkan atau mempertahankan aktivitas antioksidan (Wijayanti et al, 2017). Berdasarkan penelitian Sukrama, (2015), teh kombucha dapat berpotensi menurunkan kadar asam urat pada tikus melalui penghambatan xantin oksidase dan penurunan kada MDA (Malondialdehid) yang terbentuk selama proses pembentukan asam urat. Teh kombucha merupakan produk olahan hasil fermentasi oleh khamir dan bakteri asam asetat pada media yang mengandung gula (Naland, 2004).…”

“…Selain senyawa flavonoid terdapat senyawa metabolit sekunder lainnya yang memiliki aktivitas antihiperurisemia akan tetapi diperlukan penelitian lebih lanjut mengenai senyawa lainnya yang dapat berpotensi sebagai antihiperurisemia. Berdasarkan penelitian Sukrama, (2015), teh kombucha dapat berpotensi menurunkan kadar asam urat pada tikus melalui penghambatan xantin oksidase dan penurunan kada MDA (Malondialdehid) yang terbentuk selama proses pembentukan asam urat. Mycek, M. J., Harvey, R. A., Champe, P. C., & Fisher, B.…”