Xiao-Qing-Long-Tang shows preventive effect of asthma in an allergic asthma mouse model through neurotrophin regulation

Abstract

BackgroundThis study investigates the effect of Xiao-Qing-Long-Tang (XQLT) on neurotrophin in an established mouse model of Dermatophagoides pteronyssinus (Der p)-induced acute allergic asthma and in a LA4 cell line model of lung adenoma. The effects of XQLT on the regulation of nerve growth factor (NGF) and brain-derived neurotrophic factor (BDNF), airway hyper-responsiveness (AHR) and immunoglobulin E were measured.MethodsLA4 cells were stimulated with 100 μg/ml Der p 24 h and the supernatant was collected for ELISA analysis. Der p-stimulated LA4 cells with either XQLT pre-treatment or XQLT co-treatment were used to evaluate the XQLT effect on neurotrophin.Balb/c mice were sensitized on days 0 and 7 with a base-tail injection of 50 μg Dermatophagoides pteronyssinus (Der p) that was emulsified in 50 μl incomplete Freund’s adjuvant (IFA). On day 14, mice received an intra-tracheal challenge of 50 μl Der p (2 mg/ml). XQLT (1g/Kg) was administered orally to mice either on days 2, 4, 6, 8, 10 and 12 as a preventive strategy or on day 15 as a therapeutic strategy.ResultsXQLT inhibited expression of those NGF, BDNF and thymus-and activation-regulated cytokine (TARC) in LA4 cells that were subjected to a Der p allergen. Both preventive and therapeutic treatments with XQLT in mice reduced AHR. Preventive treatment with XQLT markedly decreased NGF in broncho-alveolar lavage fluids (BALF) and BDNF in serum, whereas therapeutic treatment reduced only serum BDNF level. The reduced NGF levels corresponded to a decrease in AHR by XQLT treatment. Reduced BALF NGF and TARC and serum BDNF levels may have been responsible for decreased eosinophil infiltration into lung tissue. Immunohistochemistry showed that p75NTR and TrkA levels were reduced in the lungs of mice under both XQLT treatment protocols, and this reduction may have been correlated with the prevention of the asthmatic reaction by XQLT.ConclusionXQLT alleviated allergic inflammation including AHR, IgE elevation and eosinophil infiltration in Der p stimulated mice by regulating neurotrophin and reducing TARC. These results revealed the potential pharmacological targets on which the XQLT decotion exerts preventive and therapeutic effects in an allergic asthma mouse model.

Val66Met polymorphism in the BDNF gene in children with bronchial asthma

“…Several studies also demonstrated the role of BDNF and other neurotrophins in the other chronic respiratory diseases, for example, chronic obstructive pulmonary disease . Moreover, the positive therapeutic effect of different anti‐inflammatory antiasthmatic medicaments is probably mediated through the neurotrophins regulation . A recent study has demonstrated that the monotherapy with salmeterol increased BDNF concentration in serum and platelets which may explain the adverse effect of such monotherapy on the asthmatic airways.…”

Alterations of p75 neurotrophin receptor and Myelin transcription factor 1 in the hippocampus of perinatal phencyclidine treated rats

“…Lingo-1 (83 kDa) 36 , NgR (51 kDa) 37 , p75 (75 kDa) 38 , TROY (46 kDa) 39 , WNK1 (250 kDa) 31 , and Myt1 (135 kDa) 31 ( Figure 1). 8 …”

Efficacy and safety of So-Cheong-Ryong-Tang in treatment of perennial allergic rhinitis: study protocol for a double-blind, randomised, parallel-group, multicentre trial

“…7 8 Another study reported that SCRT exerts a preventive effect against asthma via regulation of neutrotrophin in an allergy-based asthma disease model. 29 In previous clinical studies, Bu-zhong-yi-qi-tang displayed anti-inflammatory activity, suppressing total serum IgE and the IL-4-stimulated production of prostaglandin E2 and leukotriene C4 by polymorphonuclear neutrophils in patients with PAR. 30 In another clinical study, Xin-yi-san was demonstrated to exert an antiallergic effect by enhancing IL-0 and IL-8 production.…”