Yeast extract induction of sanguinarine biosynthesis is partially dependent on the octadecanoic acid pathway in cell cultures of Argemone mexicana L., the Mexican poppy

Guízar‐González, Cecilia; Monforte‐González, Miriam; Vázquez‐Flota, Felipe

doi:10.1007/s10529-016-2095-2

Cited by 11 publications

(6 citation statements)

References 16 publications

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“…Moreover, no preferential expression of any of the NCS or BBE isoforms was noticed in any of the tissues (Figure 3), what suggests that both isoforms might function either as redundant or additive copies, rather than being associated to a differential tissuespecific expression. No differential expression of these isoforms had previously been noticed neither in developing A. mexicana seedlings when exposed to diverse conditions, nor in vitro cultures exposed to a set of chemical elicitors (Guízar-González et al, 2016;Xool-Tamayo et al, 2017a, b), which supports this interpretation.…”

Section: Discussionsupporting

confidence: 58%

TISSUE DISTRIBUTION OF TRANSCRIPTS INVOLVED IN BIOSYNTHESIS OF BENZYLISOQUINOLINE ALKALOID IN MATURE PLANTS OF Argemone mexicana L. (Papaveraceae)

et al. 2018

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The distribution of berberine and sanguinarine was analyzed in roots, stems and leaves of mature Argemone mexicana plants, along with that of transcripts corresponding to selected genes involved in both early biosynthetic reactions, which are common to both alkaloids, and in the late specific reactions conducting to the formation of each of them. Roots were the main sites of alkaloid accumulation, though they showed the lowest accumulation of the analyzed transcripts. Results are discussed in terms of the operation of a possible transport mechanism of alkaloids between the aerial tissues and the roots, or the occurrence of different biosynthetic alternative reactions in both parts, aerial and underground tissues, involving different gene products, yet with similar catalytic capacities.

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Section: Discussionsupporting

confidence: 58%

TISSUE DISTRIBUTION OF TRANSCRIPTS INVOLVED IN BIOSYNTHESIS OF BENZYLISOQUINOLINE ALKALOID IN MATURE PLANTS OF Argemone mexicana L. (Papaveraceae)

et al. 2018

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“…Yeast extract is one of the most common natural elicitors used in in vitro culture to induce secondary metabolites production (Cheng et al 2013;Karalija et al 2020). Yeast extract has been used successfully in various studies to improve the production of secondary metabolites such as rosmarinic acid (Gonçalves et al 2019), sanguinarine (Guízar-González et al 2016), xanthone (Krstić-Milošević et al 2017) iso avonoid (Rani et al 2020), plumbagin (Singh et al 2020) and astragalosides (Park et al 2020).…”

Section: Introductionmentioning

confidence: 99%

Improvement and prediction of secondary metabolites production under yeast extract elicitation of Azadirachta indica cell suspension culture using response surface methodology

Farjaminezhad

Garoosi

2021

Preprint

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Neem is the medicinal plant used as antimalarial, antibacterial, antiviral, insecticide, and antimicrobial. This study aimed to investigate and prediction the effect of yeast extract and sampling time on cell growth, secondary metabolites synthesis, SQS1 and MOF1 genes expression by response surface methodology. The highest fresh and dry cell weights were 580.25 g/L and 21.01 g/L obtained 6 days after using 100 mg/L yeast extract. The highest azadirachtin accumulation and production were 16.08 mg/g DW and 219.78 mg/L obtained 2 and 4 days after using 25 mg/L yeast extract. Maximum mevalonic acid accumulation and production were 1.75 mg/g DW and 23.77 mg/L observed 2 days after application of 50 mg/L yeast extract. The highest amount of squalene accumulation (0.22 mg/g DW) and production (4.53 mg/L) were achieved 4 days after using 50 mg/L yeast extract. Prediction results showed that the highest azadirachtin accumulation (13.61 mg/g DW) and production (190.50 mg/L), mevalonic acid accumulation (0.50 mg/g DW) and production (5.57 mg/L), and squalene accumulation (0.30 mg/g DW) obtained by using 245 mg/L yeast extract for 2 days, 71 mg/L yeast extract for 2 days, 200 mg/L yeast extract for 4.96 days, without yeast extract for 6.54 days and 4 days, respectively. Also, predicted that the highest squalene production is achieved by long-term exposure to high concentrations of yeast extract. The qRT-PCR analysis showed the maximum relative gene expression of SQS1 and MOF1 obtained by using 150 mg/L yeast extract for 4 days and 25 mg/L yeast extract for 2 days, respectively.

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“…In contrast, SA has a greater effect on the P6H gene than on DBOX. In Argemone mexicana, yeast extract increases DBOX activity, but does not increase after MeJa induction 58 . This shows that different species are affected differently by the elicitor.…”

Section: Discussionmentioning

confidence: 83%

Influence of different elicitors on BIA production in Macleaya cordata

Huang

Xia

Zhou

et al. 2021

Sci Rep

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Sanguinarine (SAN) and chelerythrine (CHE) have been widely used as substitutes for antibiotics for decades. For a long time, SAN and CHE have been extracted from mainly Macleaya cordata, a plant species that is a traditional herb in China and belongs to the Papaveraceae family. However, with the sharp increase in demand for SAN and CHE, it is necessary to develop a new method to enhance the supply of raw materials. Here, we used methyl jasmonate (MJ), salicylic acid (SA) and wounding alone and in combination to stimulate aseptic seedlings of M. cordata at 0 h, 24 h, 72 h and 120 h and then compared the differences in metabolic profiles and gene expression. Ultimately, we found that the effect of using MJ alone was the best treatment, with the contents of SAN and CHE increasing by 10- and 14-fold, respectively. However, the increased SAN and CHE contents in response to combined wounding and MJ were less than those for induced by the treatment with MJ alone. Additionally, after MJ treatment, SAN and CHE biosynthetic pathway genes, such as those encoding the protopine 6-hydroxylase and dihydrobenzophenanthridine oxidase enzymes, were highly expressed, which is consistent with the accumulation of SAN and CHE. At the same time, we have also studied the changes in the content of synthetic intermediates of SAN and CHE after elicitor induction. This study is the first systematic research report about using elicitors to increase the SAN and CHE in Macleaya cordata.

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Yeast extract induction of sanguinarine biosynthesis is partially dependent on the octadecanoic acid pathway in cell cultures of Argemone mexicana L., the Mexican poppy

Abstract: Results suggest partial involvement of OCDA pathway in this response.

Cited by 11 publications

References 16 publications

TISSUE DISTRIBUTION OF TRANSCRIPTS INVOLVED IN BIOSYNTHESIS OF BENZYLISOQUINOLINE ALKALOID IN MATURE PLANTS OF Argemone mexicana L. (Papaveraceae)

TISSUE DISTRIBUTION OF TRANSCRIPTS INVOLVED IN BIOSYNTHESIS OF BENZYLISOQUINOLINE ALKALOID IN MATURE PLANTS OF Argemone mexicana L. (Papaveraceae)

Improvement and prediction of secondary metabolites production under yeast extract elicitation of Azadirachta indica cell suspension culture using response surface methodology

Influence of different elicitors on BIA production in Macleaya cordata

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