Yeast lactic dehydrogenase and cytochrome b2

Abstract

HIPPURYLGLYCERIDE 229 activity of the extract was such that 75 % of added ethyl butyrate was hydrolyzed in 3-5 hr. incubation time. SUMMARY 1. The synthesis of an ester of glycerol with hippuric acid from glycerol chlorohydrin and sodium hippurate is described. 2. Cleavage effected by the action of a glycerol extract of pancreatin activated by sodium taurocholate amounted to 32-6 % after 270 min. The cleavage by the same extract in the absence of sodium taurocholate was 27-2 %. 3. Cleavage of hippurylglyceride was not effected by liver esterase.

A comparative study of the succinic dehydrogenase-cytochrome system in heart muscle and in kidney

“…20% trichloroacetic acid added. The flocculent precipitate was * The validity of this assumption is supported by the finding of Bach, Dixon & Zerfas (1946) that the absorption coefficient ofthe oc band of cytochrome b2 is ofthe same order as that of cytochrome c.…”

“…The suggestion has been made by Bach et al (1946) and by Ball, Anfinsen & Cooper (1947) that cytochrome b and succinic dehydrogenase are identical. To avoid confusion, it is necessary to distinguish between two meanings which are given to the term 'succinic dehydrogenase '.…”

Principles and Procedures in the Isolation of Enzymes

“…Specific directions for its preparation are available (1 1,25,30,107,109). Among many enzyme purifications to which it has been applied quite recently may be mentioned L-amino acid oxidase (ll), hexokinase (26)' and lactic dehydrogenase (27). Other adsorbents which have been used are the alumina hydrates (7,28,110), bentonites (28,85,108), various silicas (27,41), and less frequently, a wide variety of other inorganic adsorbents (1).…”

A respiratory catalyst required for the reduction of cytochrome c by cytochrome b

“…Another dehydrogenase which, like succinic dehydrogenase, reacts with the cytochromes without the intervention of the nicotinamide nucleotides or diaphorase, is the lactic dehydrogenase of yeast which is probably identical with cytochrome b2, a haematin compound, whose spectrum is not visible in yeast, but which was obtained in a concentrated form by Bach et al (1946). These authors showed that the rapid reduction of cytochrome c by cytochrome b2 required an additional factor (X), which is removed in the purification and which is not required for the reduction of methylene blue.…”

“…(It is possible that succinic dehydrogenase is identical with cytochrome b. See Bach, Dixon & Zerfas, 1946;Ball, Anfinsen & Cooper, 1947;Slater, 1949a. ) Similarly, the activity of the cytochrome a-cytochrome a3 portion (henceforth referred to as cytochrome oxidase) may be measured by determining the rate of oxidation, in the presence of excess cytochrome c, of a number of substances, such as p-phenylenediamine, ascorbic acid and quinol, which rapidly reduce cytochrome c. * Calculated from Borsook & Schott (1931).…”