Zero dead volume flow cell for microbore liquid chromatography with Fourier transform infrared spectrometric detection

Johnson, Charles C.; Taylor, Larry T.

doi:10.1021/ac00278a006

Cited by 48 publications

(11 citation statements)

References 28 publications

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“…Johnson and Taylor [84] introduced a cylindrical flow cell with zero dead volume and reported improved detection limits over parallel plate cells. Johnson and Taylor [84] introduced a cylindrical flow cell with zero dead volume and reported improved detection limits over parallel plate cells.…”

Section: Flow Cellsmentioning

confidence: 99%

Other Modes of Detection

Parriott

1993

A Practical Guide to HPLC Detection

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Section: Flow Cellsmentioning

confidence: 99%

Other Modes of Detection

Parriott

1993

A Practical Guide to HPLC Detection

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“…[20][21][22][23][24][25] Transmission spectroscopy in an IR-element was also reported previously. 26,27 Lambrecht et al showed the potential of a QCL-based determination of glucose by means of fiber-based evanescent field spectroscopy. 28 In addition, first fiber-based transmission measurements were reported in this reference.…”

Section: Introductionmentioning

confidence: 99%

Continuous glucose monitoring by means of mid-infrared transmission laser spectroscopy in vitro

Vrančić

Fomichova

Gretz

et al. 2011

Analyst

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The continuous surveillance of glucose concentration reduces short-term risks and long-term complications for people with diabetes mellitus, a disorder of glucose metabolism. As a first step towards the continuous monitoring of glucose, reagent-free transmission spectroscopy in the mid-infrared region has been carried out in vitro using a quantum cascade laser and an optical silver halide fiber. A 30 μm gap in the fiber allowed for transmission spectroscopy of aqueous glucose solutions at a wavelength of 9.69 μm, which is specific to a molecular vibration of glucose. A noise-equivalent concentration as low as 4 mg/dL was achieved at an average power of 1.8 mW and an integration time of 50 s. This is among the most precise of glucose measurements using mid-infrared spectroscopy. Even with the very low average laser power of 0.07 mW the sensitivity of previous results (using a fiber optical evanescent field analysis) has been improved upon by almost one order of magnitude. Finally, the impact of potentially interfering substances such as other carbohydrates was analyzed.

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“…The success or failure of the technique almost exclusively depends on the interface performance. In general, flow cells [1][2][3] and solvent elimination [4][5][6][7][8][9][10][11][12][13][14][15][16][17][18] procedures have been used for the interfacing of HPLC to FTIR. Although more convenient, the usefulness of the flow-cell procedure is limited because the aqueous-based mobile phase, commonly used in reversed phase (RP)-HPLC, strongly absorbs IR radiation in many areas of the IR spectrum, resulting in a loss of sample spectral information.…”

Section: Introductionmentioning

confidence: 99%

Enhanced Desolvation and Improved Deposition Efficiency by Modified Thermospray for the Coupling of HPLC and FT-IR

Mottaleb

Kim

2002

ANAL. SCI.

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The interface is a critical component and plays a dominant role in combining HPLC and FTIR spectrometry. The success or failure of the technique almost exclusively depends on the interface performance. In general, flow cells 1-3 and solvent elimination [4][5][6][7][8][9][10][11][12][13][14][15][16][17][18] procedures have been used for the interfacing of HPLC to FTIR. Although more convenient, the usefulness of the flow-cell procedure is limited because the aqueous-based mobile phase, commonly used in reversed phase (RP)-HPLC, strongly absorbs IR radiation in many areas of the IR spectrum, resulting in a loss of sample spectral information. More favorable results can be obtained if a solvent-elimination technique is applied prior to FTIR detection.Wood 4 demonstrated the suitability of particle-beam HPLC-FTIR by depositing column effluents onto a KBr substrate from a normal-phase (NP)-HPLC solvent. This technique was only open to an NP-HPLC eluent, where non-aqueous mobile phase solvents were used. Fujimoto et al. 5 employed stainless-steel wire nets (SSWN) in lieu of applying water-soluble metal halide substrates for RP-HPLC. Solvents were evaporated using a heated nitrogen gas flow, and solutes were trapped in the metal nets. Although the major advantage of the procedure was the insolubility of SSWN in the aqueous phase, it was operated at an extremely low flow rate of 4 µl min -1 . A narrow-bore RP-HPLC-FTIR method was developed by Gagel and Biemann. 6Effluents were continuously deposited onto and evaporated from the surface of a reflective disk using a nitrogen gas nebulizer. The system offered good sensitivity (31 ng for quinoline) and could be used with up to 50% water content at a flow rate of 30 µl min -1 . A commercial version of this interface, the LC-Transform, was applied to determine polymers 7 and steroids. 8 The LC-Transform differs from Gagel and Biemann's system in that the solutes are deposited on the top of a germanium disk with an aluminum coating underneath to increase the absorption by the solutes. However, problems were encountered with both systems when the effluent contained nonvolatile solutes, such as phosphate buffers, which tended to accumulate on the disk.A nebulizing gas, mono-disperse aerosol generation HPLC to the FTIR interface, was developed by Robertson et al. 9,10 The system was capable of vaporizing 100% water at a flow rate of 300 µl min -1 . Although higher concentrations of volatile buffers were used by this interface, only about a 10% deposition efficiency was reported. This system was employed for determining methyl red 9 and caffeine 10 with detection limits of 100 ng and 13 µg, respectively. Griffiths and co-workers 11,12 described an HPLC-FTIR interface based on a concentric flow nebulizer, in which the mobile phase was continuously evaporated as the solutes were deposited onto a ZnSe window. This interface was suitable for NP-and RP-HPLC solvents, including eluents containing low concentrations of volatile buffers. Jansen 13 demonstrated a thermospray interface, whi...

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Zero dead volume flow cell for microbore liquid chromatography with Fourier transform infrared spectrometric detection

Cited by 48 publications

References 28 publications

Other Modes of Detection

Other Modes of Detection

Continuous glucose monitoring by means of mid-infrared transmission laser spectroscopy in vitro

Enhanced Desolvation and Improved Deposition Efficiency by Modified Thermospray for the Coupling of HPLC and FT-IR

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