Zhongshania aliphaticivorans sp. nov., an aliphatic hydrocarbon-degrading bacterium isolated from marine sediment, and transfer of Spongiibacter borealis Jang et al. 2011 to the genus Zhongshania as Zhongshania borealis comb. nov.

Abstract

A Gram-staining-negative, facultatively aerobic bacterium, designated SM-2T, was isolated from a sea-tidal flat of Yellow Sea, South Korea. Cells were catalase- and oxidase-positive motile rods with a single polar flagellum. Growth of strain SM-2T was observed at 10–37 °C (optimum, 25–30 °C), at pH 5.5–8.5 (optimum, pH 7.0–7.5) and in the presence of 0–11 % (w/v) NaCl (optimum, 2 %). Strain SM-2T contained ubiquinone-8 (Q-8) as the sole isoprenoid quinone and C17 : 1ω8c, summed feature 3 (comprising C16 : 1ω7c and/or iso-C15 : 0 2-OH), C17 : 0 and C18 : 1ω7c as the major fatty acids. Phosphatidylethanolamine, phosphatidylglycerol, diphosphatidylglycerol and an unidentified lipid were identified as the major cellular polar lipids. The G+C content of the genomic DNA was 52.2 mol%. Phylogenetic analysis based on 16S rRNA gene sequences showed that strain SM-2T formed a tight phyletic lineage with Zhongshania antarctica ZS5-23T, Zhongshania guokunii ZS6-22T and Spongiibacter borealis CL-AS9T, but that S. borealis CL-AS9T was distinct from other species of the genus Spongiibacter . Based on 16S rRNA gene sequence similarities, strain SM-2T was most closely related to S. borealis CL-AS9T, Z. antarctica ZS5-23T and Z. guokunii ZS6-22T, with similarities of 99.5 %, 98.9 % and 98.7 %, respectively, but the DNA–DNA hybridization values among these species were clearly lower than 70 %. On the basis of chemotaxonomic data and molecular properties, we propose strain SM-2T represents a novel species of the genus Zhongshania with the name Zhongshania aliphaticivorans sp. nov. (type strain SM-2T = KACC 18120T = JCM 30138T). We also propose the transfer of Spongiibacter borealis Jang et al. 2011 to the genus Zhongshania as Zhongshania borealis comb. nov. (type strain CL-AS9T = KCCM 90094T = JCM 17304T).

Spongiibacter taiwanensis sp. nov., a marine bacterium isolated from aged seawater

“…A second species of this genus, Spongiibacter tropicus, was isolated from a Synechococcus culture (Hwang & Cho, 2009). The third member of the genus, Spongiibacter borealis, proposed by Jang et al (2011), was recently reclassified as a species of the genus Zhongshania (Lo et al, 2014). Moreover, Melitea salexigens, a novel genus and species proposed by Urios et al (2008), was proven to be a later heterotypic synonym of Spongiibacter marinus (Jang et al, 2011).…”

ZHO-1, an intrinsic MBL from the environmental Gram-negative species Zhongshania aliphaticivorans

“…Z. aliphaticivorans SM-2 T is an environmental species recovered in 2013 from sea tidal flats in the Dangjin coastal area of South Korea. 9 Members of the Zhongshania genus are Gram-negative, oxidase-positive bacilli, all identified from a marine environment. ZHO-1 showed its ability to hydrolyse most b-lactams including carbapenems, but sparing aztreonam and cefepime.…”

Litorivivens lipolytica gen. nov., sp. nov., a lipolytic bacterium isolated from a tidal flat

“…The fatty acid profile of strain HJTF-7 T was clearly distinguishable from those of the type strains of two species of the genus Spongiibacter , and Z. borealis , by the differences in the proportions of branched and unsaturated fatty acids (). The fatty acid profile of strain HJTF-7 T was also distinguishable from those of the type strains of the other species of the genus Zhongshania by the differences in the proportions of branched and unsaturated fatty acids (Li et al , 2011; LO et al , 2014). The major polar lipids detected in strain HJTF-7 T were phosphatidylethanolamine and phosphatidylglycerol (Fig.…”