Zinc accumulation and utilization by wine yeasts

Nicola, Raffaele De; Hall, Nichola; Bollag, Tatiana; Thermogiannis, Georgios; Walker, Graeme M.

doi:10.2147/ijwr.s4570

Cited by 3 publications

(1 citation statement)

References 24 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…However, the present study showed that despite the fact that the fermentation cultures were centrifuged and eluted with distilled water repeatedly (more than seven times), the unbound inorganic iron of the fermentation cultures was not completely eliminated. Results from Nicola et al (2009) showed that cell walls were washed seven times with deionized and distilled water to remove any unbound inorganic zinc, but the protoplast fragments and the uptake of zinc and the association of zinc was loose and it was readily released to the medium. Therefore, in the present study, an improved dialysis bag method was used to remove iron until inorganic iron was no longer detected.…”

Section: Distribution Of Iron In Cellsmentioning

confidence: 99%

Screening of iron-enriched fungus from natural environment and evaluation of organically bound iron bioavailability in rats

Zhang¹,

Peng²,

Li³

et al. 2015

Food Sci. Technol (Campinas)

View full text Add to dashboard Cite

Iron is an essential element for nearly all living organisms, and its deficiency is the most common form of malnutrition in the world. The organic forms of trace elements are considered more bioavailable than the inorganic forms. Although Saccharomyces cerevisiae can enrich metal elements and convert inorganic iron to organic species, its tolerability and transforming capacity are limited. The aim of this study was to screen higher biomass and other iron-enriched fungi strains besides Saccharomyces cerevisiae from the natural environment. A PDA medium containing 800 μg/mL iron was used for initial screening. Fifty strains that tolerated high iron concentration were isolated from the natural environment, and only one strain, No.BY1109, grew well at Fe (II) concentration of 10,000μg/ml. According to morphological characterization, 18S rDNA sequence analysis, and biophysical and biochemical characterization, the strain No.BY1109 was identified as Rhodotorula. The iron content of No.BY1109 (10 mg Fe/g dry cell) was determined using atomic absorption spectrometry. The results of distribution of iron in the cells showed that iron ion was mainly chelated in the cell walls and vacuoles. The bioavailability in rats confirmed that strain No.BY1109 had higher absorption efficiency than that of ferrous sulfate after single dose oral administration. The present study introduces new iron supplements, and it is a basis for finding new iron supplements from natural environment.Keywords: iron-rich fungus; screening and isolation; identification; cell-iron distribution; bioavailability.Practical Application: Iron is an essential element for nearly all living organisms, and its deficiency is the most common form of malnutrition in the world. The present study described the higher bioavailability of iron from natural environment grown in high iron medium. The result is interesting and shows some new data for finding new iron supplements from natural environment.

show abstract

Section: Distribution Of Iron In Cellsmentioning

confidence: 99%

Screening of iron-enriched fungus from natural environment and evaluation of organically bound iron bioavailability in rats

Zhang¹,

Peng²,

Li³

et al. 2015

Food Sci. Technol (Campinas)

View full text Add to dashboard Cite

show abstract

Comprehensive Review on Potential Contamination in Fuel Ethanol Production with Proposed Specific Guideline Criteria

et al. 2022

View full text Add to dashboard Cite

Ethanol is a promising biofuel that can replace fossil fuel, mitigate greenhouse gas (GHG) emissions, and represent a renewable building block for biochemical production. Ethanol can be produced from various feedstocks. First-generation ethanol is mainly produced from sugar- and starch-containing feedstocks. For second-generation ethanol, lignocellulosic biomass is used as a feedstock. Typically, ethanol production contains four major steps, including the conversion of feedstock, fermentation, ethanol recovery, and ethanol storage. Each feedstock requires different procedures for its conversion to fermentable sugar. Lignocellulosic biomass requires extra pretreatment compared to sugar and starch feedstocks to disrupt the structure and improve enzymatic hydrolysis efficiency. Many pretreatment methods are available such as physical, chemical, physicochemical, and biological methods. However, the greatest concern regarding the pretreatment process is inhibitor formation, which might retard enzymatic hydrolysis and fermentation. The main inhibitors are furan derivatives, aromatic compounds, and organic acids. Actions to minimize the effects of inhibitors, detoxification, changing fermentation strategies, and metabolic engineering can subsequently be conducted. In addition to the inhibitors from pretreatment, chemicals used during the pretreatment and fermentation of byproducts may remain in the final product if they are not removed by ethanol distillation and dehydration. Maintaining the quality of ethanol during storage is another concerning issue. Initial impurities of ethanol being stored and its nature, including hygroscopic, high oxygen and carbon dioxide solubility, influence chemical reactions during the storage period and change ethanol’s characteristics (e.g., water content, ethanol content, acidity, pH, and electrical conductivity). During ethanol storage periods, nitrogen blanketing and corrosion inhibitors can be applied to reduce the quality degradation rate, the selection of which depends on several factors, such as cost and storage duration. This review article sheds light on the techniques of control used in ethanol fuel production, and also includes specific guidelines to control ethanol quality during production and the storage period in order to preserve ethanol production from first-generation to second-generation feedstock. Finally, the understanding of impurity/inhibitor formation and controlled strategies is crucial. These need to be considered when driving higher ethanol blending mandates in the short term, utilizing ethanol as a renewable building block for chemicals, or adopting ethanol as a hydrogen carrier for the long-term future, as has been recommended.

show abstract

Production of zinc-enriched biomass of Saccharomyces cerevisiae

Azad¹,

Shariatmadari²,

Torshizi³

2014

J. Elem.

View full text Add to dashboard Cite

Zinc accumulation and the growth of Saccharomyces cerevisiae were investigated in a culture with zinc sulfate-supplemented medium. The cultivations were performed on Sabouraud dextrose broth medium in aerobic conditions, without the addition of zinc (control culture) and with the addition of zinc sulfate (5, 10, 15, 30 and 60 mg ZnSO 4 l-1 medium) at 28°C for 72 hours. The results showed similar trends of yeast growth rates at 24, 48, and 72-hour interval, with concentrations above 10 mg l-1 ZnSO 4 in the nutritional medium significantly decreasing the yeast growth rate and the biomass yield (P<0.05). Substantial differences between the initial ZnSO 4 concentrations in the growth medium were demonstrated in the overall adsorption of Zn ions (Zn 2+) in yeast cells by a colorimetric assay (P<0.05). Similarly, the content of total accumulated zinc, as well as the fractions of Zn present in cells depended mainly on the zinc concentration in the medium, as the total Zn accumulation and organically bound Zn fractions were increased by elevating the ZnSO 4 supplementation in the culture medium up to 30 mg l-1 , but gradually reduced by any further addition of ZnSO 4 determined by an ICP-MASS assay (P<0.05). In the presence of 30 mg l-1 ZnSO 4 , the Zn content in the biomass increased by 24-fold, to 4132.34 mg g-1 in comparison to 171.9 mg g-1 achieved in the basal medium. Thus, the ability of S. cerevisiae to accumulate zinc can be used for production of a zinc-rich ingredient for functional food products.

show abstract

Zinc accumulation and utilization by wine yeasts

Cited by 3 publications

References 24 publications

Screening of iron-enriched fungus from natural environment and evaluation of organically bound iron bioavailability in rats

Screening of iron-enriched fungus from natural environment and evaluation of organically bound iron bioavailability in rats

Comprehensive Review on Potential Contamination in Fuel Ethanol Production with Proposed Specific Guideline Criteria

Production of zinc-enriched biomass of Saccharomyces cerevisiae

Contact Info

Product

Resources

About