2004
DOI: 10.1093/pcp/pci015
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Zinc Transporter of Arabidopsis thaliana AtMTP1 is Localized to Vacuolar Membranes and Implicated in Zinc Homeostasis

Abstract: Cation diffusion facilitator (CDF) proteins belong to a family of heavy metal efflux transporters that might play an essential role in homeostasis and tolerance to metal ions. We investigated the subcellular localization of Arabidopsis thaliana AtMTP1, a member of the CDF family, and its physiological role in the tolerance to Zn using MTP1-deficient mutant plants. AtMTP1 was immunochemically detected as a 43 kDa protein in the vacuolar membrane fractioned by sucrose density gradient centrifugation. The express… Show more

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Cited by 275 publications
(230 citation statements)
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“…Standard procedures (Lieberherr et al, 2005) and the manufacturer's protocol (Amersham Biosciences) were used for purification. Antisera against P-ATPase ( Kobae et al, 2004), V-ATPase (Kobae et al, 2004), and BiP (Hö fte and Chrispeels, 1992) were used, respectively, as markers for the plasma membrane, tonoplast, and endoplasmic reticulum.…”
Section: Subcellular Localization Analysis and Antibodiesmentioning
confidence: 99%
“…Standard procedures (Lieberherr et al, 2005) and the manufacturer's protocol (Amersham Biosciences) were used for purification. Antisera against P-ATPase ( Kobae et al, 2004), V-ATPase (Kobae et al, 2004), and BiP (Hö fte and Chrispeels, 1992) were used, respectively, as markers for the plasma membrane, tonoplast, and endoplasmic reticulum.…”
Section: Subcellular Localization Analysis and Antibodiesmentioning
confidence: 99%
“…Indeed, Fe 2+ importers as well 4 as exporters have been identified. For Zn 2+ only the importer, MTP1, has been described [8,12,13]. It is interesting to note that Arabidopsis halleri, a typical Zn hyperaccumulator, has a constitutive high expression of a MTP1 homologue [14].…”
Section: Introductionmentioning
confidence: 99%
“…The T. caerulescens ZTP1 (Assunção et al 2001) is highly homologous to Arabidopsis thaliana MTP1 (formerly ZAT1), a member of the cation diVusion facilitator family of transporters involved in vacuolar Zn sequestration (van der Zaal et al 1999;Kobae et al 2004). The ZTP1 gene is more highly expressed in the roots and shoots of a Zn-tolerant T. caerulescens accession, suggesting that this gene may contribute to Zn tolerance (Assunção et al 2001).…”
Section: Introductionmentioning
confidence: 99%