ZmMADS47 Regulates Zein Gene Transcription through Interaction with Opaque2

Qiao, Zhenyi; Qi, Weiwei; Wang, Qian; Yin, Feng; Yang, Qing; Zhang, Nan; Wang, Shanshan; Tang, Yiming; Song, Rentao

doi:10.1371/journal.pgen.1005991

Cited by 61 publications

(62 citation statements)

References 57 publications

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“…In barley, HvVP1 interacts with HvGAMYB and BPBF and represses their DNA-binding activity (Abraham et al, 2016). Maize OPAQUE2 dimerizes with MADS47 and enhances its activation of zein genes (Qiao et al, 2016). Similarly, the well-known LAFL network of seed maturation involves formation of many hetero and homodimers (Agarwal et al, 2011; Jia et al, 2014).…”

Section: Resultsmentioning

confidence: 99%

Three Rice NAC Transcription Factors Heteromerize and Are Associated with Seed Size

et al. 2016

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NACs are plant-specific transcription factors (TFs) involved in multiple aspects of development and stress. In rice, three NAC TF encoding genes, namely ONAC020, ONAC026, and ONAC023 express specifically during seed development, at extremely high levels. They exhibit significantly strong association with seed size/weight with the sequence variations located in the upstream regulatory region. Concomitantly, their expression pattern/levels during seed development vary amongst different accessions with variation in seed size. The alterations in the promoter sequences of the three genes, amongst the five rice accessions, correlate with the expression levels to a certain extent only. In terms of transcriptional properties, the three NAC TFs can activate and/or suppress downstream genes, though to different extents. Only ONAC026 is localized to the nucleus while ONAC020 and ONAC023 are targeted to the ER and cytoplasm, respectively. Interestingly, these two proteins interact with ONAC026 and the dimers localize in the nucleus. Trans-splicing between ONAC020 and ONAC026 results in three additional forms of ONAC020. The transcriptional properties including activation, repression, subcellular localization and heterodimerization of trans-spliced forms of ONAC020 and ONAC026 are different, indicating toward their role as competitors. The analysis presented in this paper helps to conclude that the three NAC genes, which are associated with seed size, have independent as well as overlapping roles during the process and can be exploited as potential targets for crop improvement.

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Section: Resultsmentioning

confidence: 99%

Three Rice NAC Transcription Factors Heteromerize and Are Associated with Seed Size

et al. 2016

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“…However, the fulllength OsLG3b from both SLG and Nipponbare showed abolished transcriptional activation function in yeast cells, and mutants with deletions of the MADS domain from Nipponbare exhibited strong transcriptional tor functionality ( Figure 3b, (1), (2) and (7)). That is, the transactivation domain of OsLG3b is presumably inhibited in its full-length native form, implying a unique conformational regulation of the transcriptional activation domain (Qiao et al, 2016;Zhang et al, 2015). Although mutants with deletions of the C-terminal end of the C domain (amino acids 225-257; Figure 3b, (5)) had no transcriptional activity, the C domain of SLG OsLG3b showed weaker transcriptional activity than that of Nipponbare OsLG3b (Figure 3b, (3) and (6) and Figure 3c).…”

Section: Expression Pattern Of Oslg3b and Its Transcription Activatormentioning

confidence: 99%

Alternative splicing of OsLG3b controls grain length and yield in japonica rice

Miao

Zhang

et al. 2018

Plant Biotechnology Journal

125

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SummaryGrain size, one of the important components determining grain yield in rice, is controlled by the multiple quantitative trait loci (QTLs). Intensive artificial selection for grain size during domestication is evidenced in modern cultivars compared to their wild relatives. Here, we report the molecular cloning and characterization of OsLG3b, a QTL for grain length in tropical japonica rice that encodes MADS‐box transcription factor 1 (OsMADS1). Six SNPs in the OsLG3b region led to alternative splicing, which were associated with grain length in an association analysis of candidate region. Quantitative PCR analysis indicated that OsLG3b expression was higher during the panicle and seed development stages. Analysis of haplotypes and introgression regions revealed that the long‐grain allele of OsLG3b might have arisen after domestication of tropical japonica and spread to subspecies indica or temperate japonica by natural crossing and artificial selection. OsLG3b is therefore a target of human selection for adaptation to tropical regions during domestication and/or improvement of rice. Phylogenetic analysis and pedigree records showed that OsLG3b had been employed by breeders, but the gene still has much breeding potential for increasing grain length in indica. These findings will not only aid efforts to elucidate the molecular basis of grain development and domestication, but also facilitate the genetic improvement of rice yield.

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“…The O2 ORF was fused to pGADT7 at the XmaI and ClaI 861 26 sites. The yeast transactivation assay was performed as previously described 862 (Qiao et al, 2016 Dual-LUC assays using N. benthamiana plants were performed as described 871 previously (Hellens et al, 2005;Liu et al, 2008). The luciferase activity of the LCM RNA-Seq data (Zhan et al, 2015).…”

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confidence: 99%