Zooming in on the molecular mechanisms of endocytic budding by time-resolved electron microscopy

Studying the Fate of Tumor Extracellular Vesicles at High Spatiotemporal Resolution Using the Zebrafish Embryo

“…On one hand, EVs arrest at the surface of the macrophage and undergo a slow internalization that can be tracked at optimal spatiotemporal resolution (Figures 6A and 6C; Video S6B). The timing of this uptake ($ 30 s) is in the range of classical endocytosis (Figure 6A) (Idrissi and Geli, 2014;Taylor et al, 2011). On the other hand, tumor EVs are first caught by a protrusion extending from the macrophage, and then crawl back toward the cell center before being internalized at the basis of the protrusion ( Figures 6B and 6C; Video S6C).…”

Geometry of membrane fission

“…Recent developments of time resolved electron microscopy and tomography (Idrissi and Geli, 2013;Kukulski et al, 2012) in combination with rapidly developing fluorescence microscopy techniques have advanced our understanding of distinct protein moduli controlling different stages of membrane deformations preceding the neck breakup (Idrissi and Geli, 2013). The metastable neck in its optimal configuration is a very constrained membrane nanostructure.…”

Clathrin-mediated endocytosis in budding yeast at a glance

“…Many studies have focused on how the forces and geometries needed to generate membrane scission can be generated by actin and the late-arriving scission proteins (Kaksonen et al, , 2005Picco et al, 2015;and reviewed in Goode et al, 2015;Idrissi and Geli, 2014). Nevertheless, whether the membrane bends before or after actin arrives is still debated (see poster; Idrissi et al, 2012;Kukulski et al, 2012).…”