Zum Vorkommen von Sterinen in Bakterien

Abstract

Investigations concerning sterols were made in one of strain each of nine species of bacteria and one representative of the basidiomyeetes through adsorption chromatography, gas chromatography and mass spectrometry as well as ultraviolet and infrared spectroscopy.Three strains contained sterols in quantities ranging from 0.0004 to 0.01 O i 0 of the dry weight. I n Azotobacter chroococcum six sterols were demonstrated. I n six strains sterols were not detectable. The limit of detectability at present is a t 0.0001 O/ , , sterol related to 100 g dry cells.From the extract of A erobacter cloacae, which contained no sterol, a C,,-hydroxy-ketone was isolated.

Evolution, Nutrition, Intestinal Microflora, and Prevention of Cancer: A Hypothesis

“…Sterols as well as flavonoids (or at least their precursors) have been found in phylogenetically very early prokaryotic organisms like blue-green algae (cyanobacteria) (13)(14)(15)(16). It is possible that the flavones in the green algae have been a result of some mutation of systems that introduce double bonds into the rings of steroids (16).…”

The Bacterial Membrane

“…Except for trace amounts (Schubert et al, 1968;de Souza and Nes, 1968), sterols are generally believed to be absent from the lower protists (bacteria and blue-green algae), which lack the ability to biosynthesize these compounds (see Bergmann, 1962;Salton, 1968b). However, it should be noted that Levin and Bloch (1964) found small amounts (less than 1.8 X 10-4 % of wet weight of cells) in blue-green algae.…”

Occurrence of isoprenoid compounds in gram-negative methanol-, methane-, and methylamine-utilizing bacteria.

“…On the other hand, until 1967, squalene and sterols had been encountered only in eukaryotic organisms, and not in prokaryotic organisms, bacteria and blue-green algae (4). In recent years, sterols and the sterol precursor, squalene, have been detected in Azotobacter chrococcum (5), Staphylococcus aureus (6,7), Halobacterium cutirubrum (8), Methylococcus capsulatus (9,10), Bacillus acidocaldarius (I1), and Acetobacter xylinum (12), and methanol-utilizing bacteria such as Methylobacterium organophilum (13), Pseudomonas sp. C (14), "Pseudomonas methylotropha" (14), "Methylomonas me thanolica" (14), Pseudomonas sp.…”