Zur Biochemie der Verknöcherung, I. Mineralgehalt und Grundsubstanzzusammensetzung des hyalinen Knorpels, des verknöchernden Knorpels und des Knochens

Hj, Dulce

doi:10.1515/bchm2.1960.319.1.257

Cited by 25 publications

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“…The group of ribs in which calcification was well advanced showed a significant decline in the total rib CS from the amount which was present in the early stages of calcification. Similar observations were made by Eichelberger and Roma (I), and Dulce (12). Bar addition to these changes in total CS content, Tables I and 11 show that during both the early and the later stages of calcification in puppy ribs there was a significant increase in the amount of CS found in S8 (alkaline extract), and during the later stages there was a significant decrease in the amount of CS in (water extracts).…”

Section: Discussionsupporting

confidence: 82%

Chondroitin sulfate changes in puppy rib cartilage during the period of calcification

Bowness¹,

Jacobs²

1968

Can. J. Biochem.

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B O~B S S , J. M., and S~e m , M. Chohdroitin sulfate changes Im puppy rib cartilage during the period of calcification. Can. 9. Bimkm. 46, 63 (1968).Using a single litter of seven puppies, it was found that in the period (1'3-30 weeks of age) when calcification was occurring in most of the ribs there was a change in the extractability of the chondroitin sulfate in this tissue. There was a decrease in the amount of chondroith sulfate which could be extracted by homogenizing with water and an increase in the amount which was extracted subsequently by homogenization with alkali. This change was not due to binding of chondroitin sulfate to solid mineral matter because it was found also in a single mcalcified rib from an older puppy in which all but the first two anterior ribs were heavily calcified. It was also found when EDTA was us& to extract the solid mineral matter. Measurement of specific activities after injection of radioactive sulfate showed that there was little change in the relative rates of synthesis of the water-soluble and water-insoluble chondroitin sulfate fractions as the age of the puppies increased. This leaves a change in the relationship of protein to chondroitin sulfate in complexes of the two as the most likely explanation for the observed change in extractability. Iw%rduc$isn Extraction ProcedureIn order to remove all water-soluble calcium, inorganicEichelberger and Roma (1) studied the changes phosphorus, and CS, 0.5-g portions of rib cartilage slices in total calcium, phosphate, and mucopoly-were homogenized (in a fluted container using a Virtis saccharide sulfate during calcification in puppy "23" homogenizer w w d g at top speed) with 10 ml subsequently, the chondroitin sulfate of distilled water at 4 "C for 40 min. The homogenate was centrifuged at 27 000 x g and 4 "C for 30 min, and the cartilage has been to consist at least supernatant extract poured off and retained for analysis. two metabolically distinct fractions (2-4). The The precipitate was then homogenized with a further 18 fraction with the Bower turnover rate can onlv be d water and the p r~e b r e repeated to yield a total of extracted from cartilage by homogenization kith alkaline solutions and it has a greater affinity for calcium than the water-soluble chondroitin sulfate (5, 6). On ~e basis of in vitro precipitation experiments it has been suggested that the two fractions may produce a gradient in calcium concentrations in cartilage and that their calcium binding properties wodd influence the course of calcification in the tissue (7).The present work was undertaken in order to find out if there is any change in concentration of the main chondroitin sulfate (CS) fractions during the period when calcification is occurring in puppy ribs. Materials md MethodsFor the results shown in Tabbs I-111, puppies from the same litter of seven animals were idled with chloroform three water extracts (SI-~).At this stage a water-insoluble residue and three water extracts (SIy3) had been obtained. To remove the material that might be bou...

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Section: Discussionsupporting

confidence: 82%

Chondroitin sulfate changes in puppy rib cartilage during the period of calcification

Bowness¹,

Jacobs²

1968

Can. J. Biochem.

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Enhanced mineralization of the tibial epiphyseal plate in the rat following propylthiouracil treatment: A histochemical, light, and electron microscopic study

Dearden

1974

Anat. Rec.

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Following treatment with propylthiouracil, most chondrocytes in the reserve and upper proliferation zones in the tibial epiphyseal plate exhibit degenerate ultrastructural morphology. In the extracellular matrix, sulfated mucopolysaccharide is reduced, matrix vesicles are increased both in number a n d i n size, apatite crystals are present in all zones, and mineralization is enhanced.In post-treatment recovery, chondrocyte degeneration decreases; however, the extracellular matrix is considerably delayed in returning to conditions comparable to those in corresponding controls and this is indicated by the continued presence of apatite crystals in abnormal locations. Chondrocyte degeneration, sulfated mucopolysaccharide, and matrix vesicles, are discussed as they relate to the mineralization process.

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Stoffwechselstörungen des Knorpels und ihre Beziehungen zur Arthrose

Böni¹

1965

Stoffwechsel Und Degenerativer Rheumatismus

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Zur Biochemie der Verknöcherung, I. Mineralgehalt und Grundsubstanzzusammensetzung des hyalinen Knorpels, des verknöchernden Knorpels und des Knochens

Cited by 25 publications

References 0 publications

Chondroitin sulfate changes in puppy rib cartilage during the period of calcification

Chondroitin sulfate changes in puppy rib cartilage during the period of calcification

Enhanced mineralization of the tibial epiphyseal plate in the rat following propylthiouracil treatment: A histochemical, light, and electron microscopic study

Stoffwechselstörungen des Knorpels und ihre Beziehungen zur Arthrose

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