α-Amylase and Lipase Inhibitory Activity and Structural Characterization of Acacia Bark Proanthocyanidins

Kusano, Rie; Ogawa, Sosuke; Matsuo, Yosuke; Tanaka, Takashi; Yazaki, Yoshikazu; Kouno, Isao

doi:10.1021/np100372t

Cited by 127 publications

(104 citation statements)

References 53 publications

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“…All of them contain tannin. In recent years, tannin has been reported as non-specific inhibitors for several hydrolytic enzymes such as lipases, α-glucosidases, α-amylases and invertase (Barret et al, 2013;Goncalves et al, 2011;Grussu et al, 2011;Ikarashi et al, 2011;Kusano et al, 2011;Yilmazer-Musa et al, 2012;Zajacz et al, 2007). This hypothesis can be proved by further experiments such as tannin isolation and activity assay of isolate that have been separated from other compounds.…”

Section: Discussionmentioning

confidence: 95%

Antidiabetic Activity and Phytochemical Screening of Extracts from Indonesian Plants by Inhibition of Alpha Amylase, Alpha Glucosidase and Dipeptidyl Peptidase IV

Elya¹,

Handayani²,

Sauriasari³

et al. 2015

Pakistan J. of Biological Sciences

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A B S T R A C T Active compounds of plants have potency as antidiabetic that can be used in diabetes mellitus treatment. One of the strategies is maintaining postprandial glucose level through inhibition of α-amylase and α-glucosidase and preventing the hydrolysis of Glucagon Like Peptide-1 (GLP-1) through inhibition of Dipeptidyl peptidase IV. So that pre-prandial and post-prandial glucose levels can be controlled properly. The aim of this study was to determine in vitro inhibitory activity of α-glucosidase, α-amylase and Dipeptidyl peptidase IV from 42 ethanolic extracts of selected Indonesian plants. Inhibitory activity was measured using spectrophotometric method. α-amylase activity was measured at λ = 540 nm, α-glucosidase activity and the DPP-IV activity were measured at λ = 405 nm. Eight extracts inhibit both α-glucosidase and α-amylase better than acarbose as a positive control. These extracts are Camelia sinensis (white, green, fermented), Pometia pinnata, Syzygium polyanthum, Artocarpus heteropyllus, Lagerstroemia speciosa and Persea Americana. The ethanolic extracts have DPP-IV inhibitory activity between 10-20%, lower than Diprotin as positive control, except Camelia sinensis (white) which gave the inhibition more than 30% at 62.5 µg mLG 1 . This study showed us that some extracts have inhibition activity of α-glucosidase, α-amylase and DPP-IV.

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Section: Discussionmentioning

confidence: 95%

Antidiabetic Activity and Phytochemical Screening of Extracts from Indonesian Plants by Inhibition of Alpha Amylase, Alpha Glucosidase and Dipeptidyl Peptidase IV

Elya¹,

Handayani²,

Sauriasari³

et al. 2015

Pakistan J. of Biological Sciences

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“…[31][32][33][34][35][36] Among them, indigestible dextrin has been confirmed to suppress the postprandial increase in blood glucose level by inhibiting α-glucosidase 35) and it is widely used as a food for specified health use and as a functional food. It seems reasonable to consider that Ca-Alg works similarly.…”

Section: Biological and Pharmaceutical Bulletin Advance Publicationmentioning

confidence: 99%

Mechanism of Suppression of Blood Glucose Level by Calcium Alginate in Rats

Idota

Katō

Shiragami

et al. 2018

Biological & Pharmaceutical Bulletin

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“…The a-amylase (A8220, Sigma-Aldrich, St. Louis, MO) enzyme inhibitory activity was measured using the method reported by Kusano et al (2011) with slight modifications. Substrate was prepared by boiling 100 mg potato starch in 5 ml phosphate buffer (pH 7.0) for 5 min, then cooling to room temperature.…”

Section: Assay For A-amylase Inhibitionmentioning

confidence: 99%

Inhibitors ofα-glucosidase andα-amylase fromCyperus rotundus

Tran

Nguyen

et al. 2013

Pharmaceutical Biology

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Context: A methanol extract of Cyperus rotundus L. (Cyperaceae) rhizomes showed inhibitory activity against a-glucosidase and a-amylase, two enzymes involve in carbohydrate digestion. Objective: Identification of compounds from C. rotundus rhizomes responsible for the inhibition of a-glucosidase and a-amylase. Materials and methods: Compounds were identified by a phytochemical investigation using combined chromatographic and spectroscopic methods. a-glucosidase and a-amylase inhibitory activities were evaluated by in vitro enzyme inhibition assays.0 ,5,6,7,8-hexahydroxyflavane (1), together with three known stilbene dimers cassigarol E (2), scirpusin A (3) and B (4) were isolated. Compound 2 inhibited both a-glucosidase and a-amylase activities while the flavane 1 only showed effect on a-amylase, and compounds 3 and 4 were active on a-glucosidase. All four compounds showed significant 2,2-diphenyl-1-picrylhydrazyl (DPPH) scavenging activity. Discussion: The inhibitory activities against a-amylase and a-glucosidase of the C. rotundus rhizomes were reported for the first time. Stilbene dimers are considered as potent inhibitors of a-glucosidase and promising antihyperglycemic agents. Conclusion: The isolated compounds may contribute to the antidiabetic property of C. rotundus.

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α-Amylase and Lipase Inhibitory Activity and Structural Characterization of Acacia Bark Proanthocyanidins

Cited by 127 publications

References 53 publications

Antidiabetic Activity and Phytochemical Screening of Extracts from Indonesian Plants by Inhibition of Alpha Amylase, Alpha Glucosidase and Dipeptidyl Peptidase IV

Antidiabetic Activity and Phytochemical Screening of Extracts from Indonesian Plants by Inhibition of Alpha Amylase, Alpha Glucosidase and Dipeptidyl Peptidase IV

Mechanism of Suppression of Blood Glucose Level by Calcium Alginate in Rats

Inhibitors ofα-glucosidase andα-amylase fromCyperus rotundus

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