α-Amylase inhibitor in local Himalyan collections of Colocasia: Isolation, purification, characterization and selectivity towards α-amylases from various sources

The roles of starch metabolic pathways in the cold‐induced sweetening process in potatoes

“…α‐Amylase inhibitors contain an array of eight cysteine residues that form four highly conserved disulfide bonds . Most α‐amylase inhibitors inhibit amylase by forming a complex structure that blocks the active site or modifies the conformation of the enzyme, ultimately leading to a reduction in catalytic activity . The cold‐induced potato amylase inhibitor, SbAI , was cloned from S. berthaultii , and in different genotypes of potato tubers a significantly negative correlation between SbAI transcript abundance and the RS content was observed .…”

A glycoprotein α -amylase inhibitor from Withania somnifera differentially inhibits various α -amylases and affects the growth and development of Tribolium castaneum

“…Monomeric Kunitz‐type inhibitors from rice, wheat and barley have already been reported . Similarly, a 6 kDa α ‐AI from Delonix regia and a 13.9 kDa α ‐AI from Carica papaya revealed a single band on SDS‐PAGE, suggesting its monomeric nature . Other low‐molecular‐weight α ‐AIs from cowpea, sorghum and papaya were also reported …”

Insect Amylase-Plant Amylase Inhibitor Interaction is Key to Success of Transgenics Against Insectherbivory

“…These results also indicated the necessity of optimum pH for the inhibitor to act. The optimum pH of E. integricepsgut α-amylase is 6.5, which is not an optimum pH for bean inhibitors to act.AIs isolated from various crops such as wheat, Phaseolus vulgaris, Amaranthus, Maize, Tepary bean, Phaseoluscoccineus, Colocasia, pigeon pea little millet, finger millet inhibited several insect amylases [3,4]. They are quite specific to their target enzyme [5]; therefore, a proteinaceous inhibitor that inhibits the activity of one insect amylase may not have the same effect on the other insect amylases.…”