β‐eudesmol suppresses allergic reactions via inhibiting mast cell degranulation

Han, Ning; Moon, Phil‐Dong; Ryu, Ka-Jung; Jang, Jae-Bum; Kim, Hyung-Min; Jeong, Hyun‐Ja

doi:10.1111/1440-1681.12698

Cited by 27 publications

(16 citation statements)

References 56 publications

(69 reference statements)

Supporting

Mentioning

Contrasting

Order By: Relevance

“…β-eudesmol was reported to inhibit the production and expression of IL6 through activation if p38 MAPK, NK-κB and caspase-1 in activated human mast cells [36]. Besides, it also reduced serum levels of histamine, IgE, IL1b, IL4, IL5, IL13 and VEGF in the passive anaphylaxis mouse model [37]. Altogether, the results of this study and previous studies suggested that the immunomodulatory effect of AL could be sum of the activity of each component in AL.…”

Section: Discussionmentioning

confidence: 97%

A randomized placebo-controlled phase I clinical trial to evaluate the immunomodulatory activities of Atractylodes lancea (Thunb) DC. in healthy Thai subjects

Kulma

Panrit

Plengsuriyakarn

et al. 2021

BMC Complement Med Ther

View full text Add to dashboard Cite

Background Atractylodes lancea (Thunb) DC. (AL) and bioactive compounds β-eudesmol and atractylodin have been demonstrated in the in vitro and in vivo studies for their potential clinical use in cholangiocarcinoma. The study was a randomized, double-blinded, placebo-controlled phase I clinical trial to evaluate the immunomodulatory effect of AL in human subjects. Methods The modulatory effects of AL and β-eudesmol and atractylodin on TNFα and IL6 expression in PBMCs were measured using real-time PCR. Blood samples were collected from forty-eight healthy subjects following oral administration of a single or multiple dosing of capsule formulation of the standardized AL extract or placebo. Serum cytokine profiles, lymphocyte subpopulations (B lymphocytes, CD8+ cytotoxic T lymphocytes, CD4+ T-helper lymphocytes, and NK cells), and cytotoxic activity of PBMCs against the cholangiocarcinoma cell line CL-6 were evaluated using cytometric bead array (CBA) with flow cytometry analysis. Results AL extract at almost all concentrations significantly inhibited both TNFα and IL6 expression in Con A-mediated inflammation in PBMCs. β-Eudesmol at all concentrations significantly inhibited only IL6 expression. Atractylodin at the lowest concentration significantly inhibited the expression of both cytokines, while the highest concentration significantly inhibited only IL6 expression. The administration of AL at a single oral dose of 1000 mg appeared to decrease IFNγ and IL10 and increase B cell, while significantly increase NK and CD4+ and CD8+ cells. A trend of increasing (compared with placebo) in the cytotoxic activity of PBMCs at 24 h of dosing was observed. AL at multiple dosing of 1000 mg for 21 days tended to decrease the production of all cytokines, while significantly inhibited IL17A production at 24 h of dosing. In addition, a significant increase in CD4+ and CD8+ cells was observed. A trend of increase in the cytotoxic activity of PBMCs was observed at 24 h but terminated at 48 h of dosing. Conclusions The results confirm the immunomodulatory activity of AL in humans. This activity, in complementary with the direct action of AL on inducing cholangiocarcinoma cell apoptosis, suggests its potential role for CCA control. Trial registration Retrospectively registered on 17 October 2020 [Thai Clinical Trials Registry (TCTR: www.clinicaltrials.in.th) Number TCTR20201020001#].

show abstract

Section: Discussionmentioning

confidence: 97%

A randomized placebo-controlled phase I clinical trial to evaluate the immunomodulatory activities of Atractylodes lancea (Thunb) DC. in healthy Thai subjects

Kulma

Panrit

Plengsuriyakarn

et al. 2021

BMC Complement Med Ther

View full text Add to dashboard Cite

show abstract

“…To assess the changes in the level of intracellular calcium with time, HMC-1 cells (1 × 10 5 ) were pretreated with Fura-2/AM in IMDM containing 10% FBS for 30 min, and then harvested as previously described [ 50 , 51 ]. After washing twice with medium containing extracellular calcium chelator EGTA (0.5 mM), the cell suspension (1 × 10 5 cells) was seeded into a 96-well plate and pretreated with LA for 20 min.…”

Section: Methodsmentioning

confidence: 99%

Effects of Linalyl Acetate on Thymic Stromal Lymphopoietin Production in Mast Cells

et al. 2018

Self Cite

View full text Add to dashboard Cite

Thymic stromal lymphopoietin (TSLP) is an important factor responsible for the pathogenesis of allergic diseases, such as atopic dermatitis and asthma. Because linalyl acetate (LA) possesses a wide range of pharmacological properties, being antispasmodic, anti-inflammatory, and anti-hyperpigmentation, we hypothesized that LA could inhibit TSLP. Therefore, enzyme-linked immunosorbent assay, quantitative polymerase chain reaction, caspase-1 assay, Western blot analysis, fluorescent analyses of the intracellular calcium levels, and the phorbol myristate acetate (PMA)-induced edema model were used to investigate how LA inhibits the production of TSLP in HMC-1 cells. LA reduced the production and mRNA expression of TSLP in HMC-1 cells. LA also inhibited the activation of nuclear factor-κB and degradation of IκBα. PMA plus A23187 stimulation up-regulated caspase-1 activity in HMC-1 cells; however, this up-regulated caspase-1 activity was down-regulated by LA. Finally, LA decreased intracellular calcium levels in HMC-1 cells as well as PMA-induced ear swelling responses in mice. Taken together, these results suggest that LA would be beneficial to treatment of atopic and inflammatory diseases by reducing TSLP.

show abstract

“…For fluorescence measurement, fura-2/AM was added in HMC-1 cells (1 × 10 5 /mL) for 30 min. The cells then were harvested to perform the calcium assay as described previously [32].…”

Section: Calcium Assaymentioning

confidence: 99%

Effects of Resveratrol on Thymic Stromal Lymphopoietin Expression in Mast Cells

et al. 2020

Self Cite

View full text Add to dashboard Cite

Background and objectives: Cytokine thymic stromal lymphopoietin (TSLP) plays a pivotal role in the pathogenesis of atopic diseases such as atopic dermatitis, allergic rhinitis, and asthma. Resveratrol (RSV) exerts various pharmacological effects such as antioxidant, anti-inflammatory, neuroprotective, and anticancer. Although, it has been verified the beneficial effects of RSV on various subjects, the effect of RSV on thymic stromal lymphopoietin (TSLP) regulation has not been elucidated. Materials and Methods: Here, we examined how RSV regulates TSLP in HMC-1 cells. Enzyme-linked immunosorbent assay, real-time polymerase chain reaction, Western blotting, and calcium assay were performed to evaluate the effect of RSV. Results: TSLP production and mRNA expression were reduced by RSV. RSV down-regulated nuclear factor-κB activation, IκBα phosphorylation as well as activation of receptor-interacting protein2 and caspase-1 in HMC-1 cells. In addition, RSV treatment decreased the up-regulation of intracellular calcium in HMC-1 cells. Conclusions: These results suggest that RSV might be useful for the treatment of atopic diseases through blocking of TSLP.

show abstract

β‐eudesmol suppresses allergic reactions via inhibiting mast cell degranulation

Cited by 27 publications

References 56 publications

A randomized placebo-controlled phase I clinical trial to evaluate the immunomodulatory activities of Atractylodes lancea (Thunb) DC. in healthy Thai subjects

A randomized placebo-controlled phase I clinical trial to evaluate the immunomodulatory activities of Atractylodes lancea (Thunb) DC. in healthy Thai subjects

Effects of Linalyl Acetate on Thymic Stromal Lymphopoietin Production in Mast Cells

Effects of Resveratrol on Thymic Stromal Lymphopoietin Expression in Mast Cells

Contact Info

Product

Resources

About