ω-3 oil intake during weight loss in obese women results in remodelling of plasma triglyceride and fatty acids

Abstract

Previous studies have shown that a combination of weight loss and fish oil supplementation reduce cardiovascular disease and diabetes risks by increasing adiponectin and reducing triacylglyceride concentrations, while weight loss alone significantly improves insulin sensitivity and reduces inflammation. Here, a metabolomic approach, using a combination of 1H-Nuclear Magnetic Resonance spectroscopy, and gas and liquid chromatography and mass spectrometry, was employed to elucidate the metabolic changes in blood plasma following weight loss and fish oil supplementation. The intervention study was conducted over 24 weeks, with 93 female subjects randomised to one of three groups. Two groups followed a 12-week weight loss program, followed by a 12-week weight maintenance period and were randomised to fish or placebo oil capsules; a control group did not follow the weight loss program and were given placebo oil capsules. Lipid profiles changed dramatically upon fish oil intake and subtly across the two weight loss groups. While the fish oil supplementation increased the proportion of various phospholipid species, previously reported reductions in total triacylglycerides (TAGs) upon fish oil intake were shown to be driven by a reduction in a specific subset of the measured TAGs. This remodelling of triglycerides may represent further beneficial effects of fish oil supplementation.Electronic supplementary materialThe online version of this article (doi:10.1007/s11306-009-0161-7) contains supplementary material, which is available to authorized users.

NMR- and LC–MS/MS-based urine metabolomic investigation of the subacute effects of hexabromocyclododecane in mice

“…For the three experiment groups, a comparison analysis generated a PLS-DA model with R 2 Y = 0.867 and Q 2 = 0.679, displaying the faithful representation and excellent predictive capacity, respectively. A robust model was linked to a Q 2 value > 0.40 (McCombie et al 2009). In fact, this was the case for this study.…”

Transcriptomic and metabolomic signatures of an n-3 polyunsaturated fatty acids supplementation in a normolipidemic/normocholesterolemic Caucasian population

“…A study by Lankinen et al (2009) [7] found that serum bioactive lipids associated with insulin signaling and inflammatory pathways, ceramides, lysophosphatidylcholines (lysoPCs) and diacylglycerols were decreased by fatty fish intake. Furthermore, McCombie et al (2009) [8] found that fish oil supplementation in conjunction with body weight loss reduced total plasma TG content; in addition, weight loss without fish oil supplementation decreased the levels of plasma docosapentaenoic acid (22:5). Overall, only a limited number of clinical studies have been conducted on the impact of n-3 PUFA on the metabolome.…”

NMR spectroscopy – a modern analytical tool for serum analytics of lipoproteins and metabolites

“…The effect of special diets on the metabolic pattern was also examined in a study on weight loss [69] . Three test groups of 31 women each were given ω -3-fish oil or a placebo over 24 weeks.…”