Генетический Полиморфизм Редких И Малораспространенных Аборигенных Донских Генотипов  L

Ilnitskaya, Elena Tarasovna; Naumova, L. G.; Ganich, Valentina Alekseyevna; Токмаков, С. В.; Makarkina, Marina Victorovna

doi:10.35547/im.2019.21.3.002

Cited by 3 publications

(1 citation statement)

References 9 publications

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“…The results of this study showed the appearance of two centers of origin of cultivated germplasm, one in the Middle East and the other in the western part of the Mediterranean Sea. In this case, the second one gave rise to many existing Western European varieties [7][8][9]. The existence of various theoretically and practically substantiated centers of origin of plant forms suggests that the preservation of endemic relict plant forms in these centers is still possible., The territory of the Northern Caucasus and the Black Sea coast (northern regions of the Black Sea) is considered the most promising of all zones from the point of view of studying the biodiversity of grapevines [10].…”

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confidence: 99%

New finds of wild grapes of the Kuban region and their environmental and geographic characteristics

Gorbunov

Lukyanova

2023

E3S Web of Conf.

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This article presents the study results of new locations of wild forms of the Krasnodar Territory, Crimean region. Two populations in two locations were found in the slope and plain territories. In the course of the expeditions, the environmental and geographic characteristics of the habitats of wild grape forms were given, a complete description of the vegetation of the ecotopes under study was made, and the morphological and biological qualitative and quantitative indicators of grape wild forms have been studied in detail. When analyzing scientific literature data, it was found that there is not enough information about Kuban wild plants, feral forms and autochthons of grapes. The urgent need for this study arose. The research was carried out using route-reconnaissance, geobotanical, ampelographic, analytical methods. The variability of some indicators was detected to a greater or lesser extent, both at interpopulation and endogenous levels. Populations differ in the shape of the leaf blade, with leaves occurring pentagonal, deltoid, and rounded. The degree of leaf corrugation varies from weak to medium. The crown of the young shoot is slightly open or half open, with very rare pubescence, the depth of the upper lateral leaf clippings is from very small to medium.

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Section: Introductionmentioning

confidence: 99%

New finds of wild grapes of the Kuban region and their environmental and geographic characteristics

Gorbunov

Lukyanova

2023

E3S Web of Conf.

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Regulation of Basic Agricultural Indicators of Grapes Growth and Development by Shoot Load

Sirotkina

2023

Vestnik of Kazan State Agrarian University

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The studies were carried out to determine the dependence of the safety of eyes, fruiting, yield, quality of grapes, and growth vigor of plants of Krasnostop Zolotovsky variety on the load of shoots. The work was carried out in production vineyards in Rostov region in 2020-2022. The feeding area of one plant was 4.5 m2 (planting pattern 3.0×1.5 m), the shape of the bushes was long-armed, the vines were covered. We tested four norms of shoot loading - 20, 25, 30 and 35 pieces per bush. The experiment was carried out in triplicate, 10 bushes each. On average, over the years of study, with an increase in the load of shoots, the proportion of living eyes after wintering along the entire length of the fruit vine decreased from 83% in the variant with 20 shoots to 64% with a load of 35 pieces per bush. The highest proportion of fruit-bearing shoots was noted in the variant with 25 shoots in the bush structure - 80%; the value of this indicator was lower than other variants in plants with a load of 35 pieces per bush - 63%. With an increase in load from minimum to maximum in the experiment, the yield increased significantly from 6.22 to 8.44 t/ha. The mass concentration of sugars in berry juice increased with increasing load of shoots from 22.6 to 25.5 g/100 cm3. The average volume of shoots decreased from 120.1 cm3 in plants with a load of 20 shoots to 62.2 cm3 with 35 shoots in the bush structure.

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DNA-fi ngerprinting of local Аbkhazian grape variety Kachich

Ильницкая

Айба²,

Makarkina

et al. 2019

Sadovodstvo i vinogradarstvo

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The study of the indigenous gene pool of Vitis vinifera L. of diff erent zones of viticulture with the involvement of molecular genetic methods is an urgent due to the wide diversity of grape varieties. The territory of Abkhazia is a unique zone, one of the hotbeds of the emergence of cultural grapes. «Kachich» is the oldest Abkhazian grape variety, known for the quality of wines obtained from its harvest. The variety is more resistant to fungal diseases compared to popular European varieties cultivated in modern Abkhazia, and is particularly resistant to rot of berries. However, often the local population calls old bushes by the name «Kachich», which give grapes of good quality for making of red wines and not always these forms correspond to the morphological description of the variety. The aim of the research is DNA fi ngerprinting of the «Kachich» grape variety based on the analysis of polymorphism of microsatellite loci. The object of the study was the plants corresponding to the variety description, growing in the collection of the agricultural company “Wines and waters of Abkhazia” (Sukhum, Abkhazia). DNA was isolated from the young leaves of the top of the shoot of typical plants of the variety by the CTAB-method. Genotyping was carried out by polymerase chain reaction (PCR) with separation of reaction products by capillary electrophoresis. Highly polymorphic SSR-markers recommended for grapes varieties identifi cation were used for study (VVS2, VVMD5, VVMD7, VVMD25, VVMD27, VVMD28, VVMD32, VrZAG62, VrZAG79). The size of amplifi ed fragments was estimated using the automatic genetic analyzer ABI Prism 3130 and special software GeneMapper and PeakScanner. To clarify the size of amplifi ed fragments, the DNA of reference varieties was used. According to the results of microsatellite analysis, the DNA-certifi cate of the genotype of the «Kachich» grape variety was compiled: VVS2153 155 VVMD5234 240 VVMD7239 249 VVMD25239 267 VVMD27186 193 VVMD28234 248 VVMD32262 272 VrZAG62194 196 VrZAG79236 236. The resulting DNA-profi le was checked for matches in the Vitis International Variety Catalogue VIVC. No matches of the obtained DNA-profi le with any other DNA-profi le presented in the Database were found. DNA-certifi cate of genotype «Kachich» can be successfully used for varieties identifi cation of grape plants if necessary to clarify their varietals affi liation.

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Генетический Полиморфизм Редких И Малораспространенных Аборигенных Донских Генотипов L

Cited by 3 publications

References 9 publications

New finds of wild grapes of the Kuban region and their environmental and geographic characteristics

New finds of wild grapes of the Kuban region and their environmental and geographic characteristics

Regulation of Basic Agricultural Indicators of Grapes Growth and Development by Shoot Load

DNA-fi ngerprinting of local Аbkhazian grape variety Kachich

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