Гістологічна, Гістохімічна Характеристика Дванадцятипалої Кишки Курей-Бройлерів За Згодовування Кормів З Високим Вмістом Пробіотичної Добавки

Kotsyumbas, G. I.; Kostynjuk, A.; Mysiv, O.; Fedyk, Yu.

doi:10.15421/nvlvet7717

Cited by 2 publications

(1 citation statement)

References 2 publications

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“…Будь-які зміни в цій рівновазі супроводжуються функціональними порушеннями, які своєю чергою призводять до зниження продуктивності. Використання пробіотиків дає змогу уникнути дисбалансу кишечнику та загибелі молодняку (Maliar et al, 2013;Lyzogub, 2017;Kotsyumbas et al, 2017).…”

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The dynamics of humoral protection factors in broilers under the conditions of probiotic preparations application

Romanovych

2018

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The health of farm poultry depends on the balance between normal and potentially pathogenic intestinal microflora. Any changes in this equilibrium are accompanied by functional impairments, which, in turn, lead to a decrease in productivity. The use of probiotics helps to avoid imbalance of intestinal microflora and death of young animals. In this connection, it is relevant to study the effects of probiotic drugs to improve the vitality and immune function of the poultry. The purpose of the research was to clarify the effect of BPS-44 and different amounts of yeast Saccharomyces cerevisiae on the activity of the humoral level of natural resistance in chicken broilers. Experiments were carried out on broiler chicks-308 grown in the Fedyuk M farm in the Zolochiv district of Lviv oblast. The maintenance of the chickens was cellular with free access to feed and water. Technological parameters of broiler cultivation (temperature and light regime) were in accordance with the norms of ONTP-2005. Experiments were conducted in 4 groups of broiler chickens of 100 chicks in each according to the scheme: the control group was fed with the standard feed (SC) according to the existing norms recommended for the ROSS-308 cross; the experimental group I in addition to the SC received a probiotic BPS-44 (registration certificate No. 2154-04-0254-06 dated November 24, 2006), based on the production strain of bacteria Bacillus subtilis ssp. subtilis 44-p, dose 0.21 g/kg, the experimental group II – 1% yeast Saccharomyces cerévisiae; the experimental group III of chickens – 2% yeast Saccharomyces cerévisiae. Blood was taken from chickens in order to carry out immunological research at different age intervals: 11, 27, 34 and 41 days of age. Blood serum was determined by bactericidal and lysozyme activity and the content of circulating immune complexes. The stimulatory effect of BPS-44 and yeast Saccharomyces cerеvisiae in the composition of mixed fodder for broiler chickens on the dynamics of the formation of humoral protection factors in the bird organism was established. At the same time, in the conditions of use of yeast Saccharomyces cerэvisiae in the composition of mixed fodder, a higher level of indices of the humoral level of non-specific resistance was recorded, than the preparation BPS-44.

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The dynamics of humoral protection factors in broilers under the conditions of probiotic preparations application

Romanovych

2018

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Study in Vitro of Antagonistic Activity of Bacillus Isolates and Selection of Promising Probiotic Strains

Chechet¹,

Kovalenko²,

Horbatyuk³

et al. 2022

SCIVP

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The effectiveness of probiotic preparations is determined by the set of biological properties of the strains of cultures that are part of the probiotic, therefore, at the current stage of the development of biological antibacterial therapy, non-pathogenic probiotic spore-forming microorganisms, in particular bacteria of the genus Bacillus, are increasingly used to neutralize bacterial infections. The aim and tasks of the work were aimed at determining in vitro the level of antagonistic activity of isolates of Bacillus subtilis, Bacillus licheniformis and Bacillus coagulans isolated from poultry to standard test cultures of gram-negative and gram-positive microorganisms Pseudomonas aeruginosa АТСС 15442, Escherichia coli АТСС 25922, Salmonella typhimurium АТСС 29630 and Staphylococcus aureus ATSS 6538 and the selection of the most promising strains for the construction of the complex probiotic preparation "Biomagn". 27 isolates of the genus Bacillus were studied, isolated from poultry from poultry farms in Ukraine and identified to the species Bacillus subtilis — 13 strains, Bacillus licheniformis — 6 strains, Bacillus coagulans — 8 strains. Research on the determination of the level of antagonistic activity of research isolates of the genus Bacillus was carried out in vitro by two diffusion methods: the method of timed antagonism and the method of perpendicular strokes. Two promising strains of Bacillus subtilis (Bs–5 and Bs–9) were identified with very high and high levels of antagonistic activity against the indicator test cultures of microorganisms Escherichia coli ATCC 25922, Pseudomonas aeruginosa ATCC 15442, Salmonella typhimurium ATCC 29630 and Staphylococcus aureus ATCC 6538; two promising strains of Bacillus licheniformis (Bfl-1, Bfl-4) with a high antagonistic potential for action on indicator test cultures of microorganisms and one promising Bacillus coagulans (strain Bcg-5) with a medium level of antagonistic properties. The selected promising strains will be used for the construction of the complex probiotic preparation «Biomagn».

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Гістологічна, Гістохімічна Характеристика Дванадцятипалої Кишки Курей-Бройлерів За Згодовування Кормів З Високим Вмістом Пробіотичної Добавки

Cited by 2 publications

References 2 publications

The dynamics of humoral protection factors in broilers under the conditions of probiotic preparations application

The dynamics of humoral protection factors in broilers under the conditions of probiotic preparations application

Study in Vitro of Antagonistic Activity of Bacillus Isolates and Selection of Promising Probiotic Strains

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