تأثير زيت وأوراق الزيتون على فئران التجارب المصابة بأمراض الکبد الحادة

Abstract

This study aimed to investigate the effect of two levels of olive oil, olive leave and their mixture on rats suffering from acute liver disease. In addition to determine the chemical composition and phenolic compounds. Forty eight male albino rats (Sprague Dawley) used in this study. Rats divided into two main groups. First main group (6 rats) was fed on basal diet, as negative control. The second main group (42 rats) fed on basal diet and were treated with CCL4 in paraffin oil (50% v/v 4ml/kg) by a single dose subcutaneous injection to induce acute damage in the liver. After injection, AST, ALT and ALP enzymes activity were determined in the first and second main groups to insure the induction. Then the rats in the second main group were divided into seven subgroups (n = 6) according to the following: Subgroup 1: fed on normal basal diet, this group used as a (positive control group). Subgroup 2&3: fed on diet containing (3.5% and 7%) olive oil, respectively. Subgroup 4&5: fed on diet containing (3.5% and 7%) olive leave , respectively. Subgroup 6: fed on basal diet containing (3.5% olive oil and 3.5% olive leave). Subgroup 7: fed on basal diet containing (7% olive oil and 7% olive leave). The results revealed that, injected rats with CCl4 induced decrease in feed intake, BWG% and HDLc, while organs weight / body weight%, serum (cholesterol, triglycerides, LDL-c, VLDL-c, AST, ALT and ALP) increased significantly, as compared to non-injected rats (control negative group). Treating acute liver disease groups with the two levels from olive oil, olive leave and their combination improved all parameters, especially the groups which were treated with (7% olive oil and 7% olive leave) and (3.5% olive oil and 3.5% olive leave). From these results, it could be concluded that, olive oil, olive leave and the combination from them improved liver enzymes and the complication resulting from CCl4.