In banana, drought responsive gene expression profiles of drought-tolerant and sensitive genotypes remain largely unexplored. In this research, the transcriptome of drought-tolerant banana cultivar (Saba, ABB genome) and sensitive cultivar (Grand Naine, AAA genome) was monitored using mRNA-Seq under control and drought stress condition. A total of 162.36 million reads from tolerant and 126.58 million reads from sensitive libraries were produced and mapped onto the Musa acuminata genome sequence and assembled into 23,096 and 23,079 unigenes. Differential gene expression between two conditions (control and drought) showed that at least 2268 and 2963 statistically significant, functionally known, non-redundant differentially expressed genes (DEGs) from tolerant and sensitive libraries. Drought has up-regulated 991 and 1378 DEGs and down-regulated 1104 and 1585 DEGs respectively in tolerant and sensitive libraries. Among DEGs, 15.9% are coding for transcription factors (TFs) comprising 46 families and 9.5% of DEGs are constituted by protein kinases from 82 families. Most enriched DEGs are mainly involved in protein modifications, lipid metabolism, alkaloid biosynthesis, carbohydrate degradation, glycan metabolism, and biosynthesis of amino acid, cofactor, nucleotide-sugar, hormone, terpenoids and other secondary metabolites. Several, specific genotype-dependent gene expression pattern was observed for drought stress in both cultivars. A subset of 9 DEGs was confirmed using quantitative reverse transcription-PCR. These results will provide necessary information for developing drought-resilient banana plants.

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In recent times, secured routing is a major research in MANETs. The behaviour of malicious nodes in this network increases the risk of threats and induces abnormal operations in MANETs. This affects the security of data transmitted between the nodes in the network. Hence, an effective technique is needed to prevent the abnormal nodes after the process of detection. In this paper, we propose an improved Trust Detection Algorithm to increase the probability of detection and prevention of Black Hole nodes in MANETs. The proposed framework observes the behaviour of each node using various trust metrics that includes the relationship between the sensor nodes, social and service attribute trust and QoS metric trusts. The behaviour of sensor nodes is found through the communication and mobility behaviour of each node. This method avoids the black hole nodes in MANETs, when the routing is carried out with Zone Routing Protocol (ZRP). Hence, the privacy of data is retained using the proposed method. The proposed method is tested in terms of different combinations of with and without trusts. The result shows that the proposed method is effective through various QoS metrics like overall throughput, packet loss, energy consumption, trust level, false acceptance rate and missed detection rate.