These findings demonstrated, for the first time, the technical feasibility of suicidal germination for controlling S. hermonthica. Optimising structure, formulation and application protocol of germination stimulants should be the main goal for further improvement of the technology. © 2016 Society of Chemical Industry.

Summary Germination of Striga hermonthica (Del.) Bentth seeds in response to the strigol analogue GR 24 was strongly influenced by soil moisture prior to, at, or after, stimulation. Exposure of seeds to excessive soil moisture contents (70% w/w) during conditioning resulted in a low response to GR 24. Transfer of Seeds from soil at 70% moisture to a lower moisture level (47%) for 2 days or more improved the response to GR 24 (37–58% germination), white air‐drying restored germination (88%). In Gezira soil the optimum soil moisture contents for maximum response to GR 24 were 44, 38 and 40% during conditioning, stimulation and germination, respectively. The corresponding figures for the sandy soil were 20, 22 and 22%, respectively. Persistence of GR 24 was similar in both soil types, but was strongly influenced by soil moisture content. The stimulant was stable in air‐dry soil, but a rapid loss was encountered in moist soil.

Summary The activity of GR7 and Striga germination stimulant(s) from Euphorbia aegyptiaca Boiss. showed adequate persistence (6–8 days) in acidic soils (pH 5·0–6·3), but residual activity was short (1–3 days) in alkaline soils. The compounds tended to lose activity at a faster rate in the alkaline clay Gezira soil (pH 7·8), than in its sandy equivalent (pH 8·1). In solution, pH had no influence on initial activity, but residual action was reduced more rapidly by alkalinity and high temperature. However, the rate of loss of activity in solution was slower than in soils.

Sorghum is an important food, feed, and industrial crop worldwide. Parasitic weeds of the genus Striga constitute a major constraint to sorghum production, particularly in the drier parts of the world. In this study we analysed the Striga germination stimulants, strigolactones, in the root exudates of 36 sorghum genotypes and assessed Striga germination and infection. Low germination-stimulating activity and low Striga infection correlated with the exudation of low amounts of 5-deoxystrigol and high amounts of orobanchol, whereas susceptibility to Striga and high germination-stimulating activity correlated with high concentrations of 5-deoxystrigol and low concentrations of orobanchol. Marker analysis suggested that similar genetics to those previously described for the resistant sorghum variety SRN39 and the susceptible variety Shanqui Red underlie these differences. This study shows that the strigolactone profile in the root exudate of sorghum has a large impact on the level of Striga infection. High concentrations of 5-deoxystrigol result in high infection, while high concentrations of orobanchol result in low infection. This knowledge should help to optimize the use of low germination stimulant-based resistance to Striga by the selection of sorghum genotypes with strigolactone profiles that favour normal growth and development, but reduce the risk of Striga infection.

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