This study was conducted to analyze carotenoids from tomatoes by high-performance liquid chromatography using reversed-phase C18 silica having cross-linked end-capping with diode array and mass spectrometric detection. An efficient gradient elution system was developed to achieve good and reliable separation of both major and minor carotenoids as well as their isomers. Resolution of lycopene, β-carotene and their isomers was 0.91-3.97 and 1.02-2.86 with cross-linked and conventional C18 column, respectively. The % recovery for zeaxanthin, lycopene and β-carotene was found to be in the range of 89-97%. Limits of detection and quantification of 19.44 and 64.79 ng/mL for zeaxanthin, 15.6 and 52.4 ng/mL for lycopene and 8.28 and 27.61 ng/mL for β-carotene were determined. More carotenoid compounds could be separated and detected with the new method as compared with conventional C18 column. Hyphenation of HPLC with photodiode array and mass spectrometry detectors assisted in detection of tetra-dehydrocarotenoid and fatty acid diesters of xanthophylls in tomato products. Content of all-trans-lycopene, β-carotene and total carotenoid in different industrial tomatoes tested was found to range between 41.87 and 84.65, 0.89 and 1.50 and 53.22 and 112.60 µg/g fresh weight, respectively.

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Erythroderma remains a condition difficult to study and treat. We showed that a correct judgment about its cause can be based on objective histopathological criteria in up to 60% of cases. More studies are needed to try to find out further histological and/or immunohistochemical markers that could help the clinician with the erythroderma etiology diagnostic process.

661.72.88 Hemostatic resorbent polymeric materials have been synthesized from water-soluble acetylceUulose, lagochilin, and lagohirsin. The substances obtained possess an effective hemostatic action and have a water-soluble form.It is known that by adding (low-molecular-mass) physiologically active substances to the macromolecule of a highmolecular-mass compound it is possible to obtain a prolongation of their action, to decrease their toxicity, to improve their solubility, and to achieve directed transport into determined organs [1].We have previously [2] reported the results of investigations on the production of a hemostatic material from watersoluble acetylcellulose by the "inclusion" in its structure of a drug --lagoden (homogeneous mixture). Low-molecular-mass a natural compounds such as lagochilin and lagohirsin, isolated from plants of the genus Lagochilus, possess only a weak hemostati6 activity because of their insolubility in water. In order to impart solubility to them and to prolong their action, and also to create polymeric medicinal forms, we have investigated the addition of molecules of lagochilin or lagohirsin to the aldehyde groups of oxidized ,/vater-soluble acetylcellulose (OWSAC).As is known, the molecule of water-soluble acetylcellulose (WSAC), obtained by the procedure of [3], contains two functional groups, -OH and -OCOCH 3, which greatly restrict the possibility of polymer-analogous transformations. With the aim of forming in it a new reactive -HC = O group we have carried out the directed oxidation of WSAC with periodic acid, which is capable of oxidizing the two hydroxy groups present at the second and third carbon atoms of the anhydroglucose unit of WSAC [4].To confirm the formation of an aldehyde group in the WSAC macromolecule we recorded IR spectra of control and oxidized specimens. In view of the fact that the absorption band of the aldehyde group coincides with the absorption band of the acetyl group, to demonstrate the formation of aldehyde groups we recorded IR spectra of saponified samples of WSAC and OWSAC. Saponification was performed with a 0.5 N solution of sodium methanolate in anhydrous methanol [5]. The IR spectra of the OWSAC specimens revealed an absorption band relating to an aldehyde group, the intensity of which depended on the time of oxidation.The number of aldehyde groups in the product was determined by titrating an aqueous solution of the OWSAC with sodium thiosulfate. The OWSAC contained 100-110 aldehyde groups per 1000 anhydroglucoside units (AGUs) and had retained its solubility in water. A further increase in the number of aldehyde groups led to the formation of intermolecular acetal bonds, as a result of which the OWSAC became insoluble.To add lagochilin or lagohirsin to the aldehyde groups of OWSAC, a definite amount of the substance was dissolved in dimethylformamide to give a concentration of 5-6%, and lagochilin or lagohirsin was added in a ratio to the polymer of 1:4. As a water-abstracting agent we used anhydrous copper sulfate or phosphoric anhydr...

The differential diagnosis between lichenoid drug eruption (LDE) and lichen planus (LP) is difficult due to similar clinical and histological signs but important for treatment and prognosis. The purpose of this study was to propose the new diagnosis method for differentiate LDE from LP. During 2015-2018, 20 patients with confirmed LDE, 13 patients with LP and 134 controls were examined and treated at the Lenoblcenter. All enrolled patients were underwent the injection of 0.5 mL of the 2% lidocaine solution by insulin syringe into the papule with following histological examination. The formation of a blister (bulla) at the site of injection was considered a positive test result. Among LDE, 18 of 20 patients were found positive for developing blister (bulla) and two results were questionable. In 12 of 13 LP patents, bulla on the site of injection was not identified and the result of one patient was nonspecific. All