No abstract

1981. Factors influencing the frequency of callus formation among cultured rice (Oryza sativa) anthers. -Physiol. Plant. 51: 201-206.The effect of various experimental parameters upon the frequency of callus formation from cultured anthers of Oryza sativa has been investigated. Although certain medium components were found to be critical to callus formation, the concentration of these components had little effect upon the frequency of callus formation. The degree to which the callus formation frequency was influenced by cold pretreatment of the tlowers was variable. Even though plants were grown under uniform conditions and flowers containing pollen in the microspore stage of development were selected for dissection, the frequency of callus formation varied nonrandomly between flowers. In experiments with populations of flowers in a physiological and developmental state favorable to callus formation 35% of plated anthers produced callus and at least 60% of these calluses gave rise to green plants.

Background: This paper sets out to present theoretical and methodological premises for the therapeutic mode of conduct which utilizes FED and PNF improvement methods complemented by introduction of correctional re-education basing on the biofeedback mechanism.

<em>In vitro</em> culture of young <em>Secale cereale</em> spikes is described with special attention given to changes in development of the rachis. 7 mm explants were cultivated on a modified M u r a s h i g e and S k o o g (1962) medium (MS) supplemented with 2,4-D (dichlorophenoxyacetic acid), NAA (α-naph-thaleneacetic acid), IAA (β-indoleacetic acid), 2,4,5-T (trichlorophenoxyacetic acid), and ZEA (zeatin), KIN (kinetin), BAP (6-benzylaminopurine), IPA (izopentenyladenine or 2 iP) in numerous combinations and concentrations. Rachises differentiated branches with node construction in the presence of synthetic auxin. Rhizogenesis of explants was stimulated by 2,4-D and 2,4,5-T. 2,4,5-T + IPA appeared to be the best combination for callus regeneration. Many meristematic centres were found previously and combinations of NAA + KIN stimulated direct, whereas 2,4-D + ZEA indirect, differentiation of shoot elements. Development was observed from plantlets to flowering plants.