Abstract. Vanamei prawns have fast growth and can reared in high density. it has an impact on water quality deterioration and disruption of survival rates and growth. Various ways to cope has been done, one of them is with probiotic bacteria. The new strain of D2.2 bacteria is thought to be effective of ammonia utilization. Probiotics with local bacteria Bacillus sp. D2.2 is cultured on molasses technical medium to be applied semi-mass. The purpose of this study is to asses the efectivity of Bacillus sp. D2.2 inthe molasses technical medium on water quality and growth performance of vaname prawns (Litopenaeus vannamei). The research was used complete randomized design (RAL) with four treatments, A (Control), B (Application of 5 ppm Bacillus sp. D2.2 cultured in molasses technical medium), C (Application of 10 ppm Bacillus sp. D2.2 cultured in molasses technical medium), D (Application of 15 ppm Bacillus sp. D2.2 cultured in molasses technical medium) were repeated three times each. The results showed no effect on water quality and shrimp survival rate, but absolute growth (W), daily growth rate (GR) and feed conversion ratio (FCR) showed that B and C treatment had better than control.Keywords: Vaname shrimp, growth, Bacillus sp. D2.2, molasses technical medium Abstrak. Udang vanamei memiliki pertumbuhan cepat dan dapat dipelihara dengan kepadatan yang tinggi. Tingkat kepadatan yang tinggi berdampak pada penurunan kualitas air dan berakibat terganggunya tingkat kelangsungan hidup dan pertumbuhan. Berbagai cara untuk menanggulangi penurunan kualitas air telah dilakukan, salah satunya adalah dengan bakteri probiotik. Strain baru bakteri D2.2 diduga efektif dalam menanggulangi peningkatan amonia. Probiotik dengan bakteri lokal Bacillus sp. D2.2 dikultur pada media teknis molase untuk diaplikasikan secara semi-massal. Tujuan penelitian ini yaitu untuk mengujiefektivitas pemberian Bacillus sp.D2.2 pada media teknis molase terhadap kualitas air dan performa pertumbuhan udang vaname(Litopenaeus vannamei). Rancangan penelitian yang digunakan adalah rancangan acak lengkap (RAL) dengan empat perlakuan yaitu A (Kontrol), B (Aplikasi 5 ppm Bacillus sp. D2.2 yang dikultur media teknis molase), C (Aplikasi 10 ppm Bacillus sp. D2.2 yang dikultur media teknis molase), D (Aplikasi 15 ppm Bacillus sp. D2.2 yang dikultur media teknis molase) masing-masing diulang tiga kali. Hasil penelitian menunjukkan tidak adanya pengaruh terhadap kualitas air dan tingkat kelangsungan hidup udang, namun pertumbuhan mutlak (W), laju pertumbuhan harian (GR) dan feed conversion ratio (FCR) menunjukkan perlakuan B dan C memiliki nilai yang lebih baik dibandingkan kontrol.

This study aims to determine the effect of giving probiotics from (hymenachne acutigluma) silage in feed to the length of smallintestine and caeca in Pegagan ducks. The object of this research is sixty 5 months Pegagan ducks. The research design used was a Completely Randomized Design (CRD) with 5 treatments and 4 replications. There is 3 Pegagan ducks as a variable unit: P0 (Control); P1 (Ration + Probiotics with a dose 0,2%); P2 (Ration + Probiotics with a dose 0.4%); P3 (Ration + Probiotics with a dose 0.6%); P4 (Ration + Probiotics with a dose 0.8%). The observed variables were length of small intestine, length of duodenum, length of jejunum, length of ileum, and length of caeca. The results of this study indicate that administration of probiotics from (hymenachne acutigluma) silage in feed can increase the percentage of small intestine length and caeca. The conclusion of this research is the provision of probiotics from (Hymenachne acutigluma) silage with a dose 0.2% can increase the percentage of small intestine length and caeca in Pegagan ducks.