The purpose of this preliminary retrospective study was to determine the prevalence of fibroma in oral mucosa among patients attending USM dental clinic from 1/6/2006-1/6/2010. Fibroma of the oral mucosa is the most common benign tumor of the oral cavity. It is a reactive hyperplasia of fibrous connective tissue in response to local irritation or trauma. A lesion on any part of the oral mucosa have a broad differential diagnosis ranging from traumatic lesions (mucocele), neurogenic lesions (neurofibromatosis), lipoma , epithelial tumors (squamous papilloma) and inflammatory/reactive hyperplasia of soft tissue (pulp polyp). A total number of 192 patients (82 male and 110 female) who are registered in the Oral Medicine and Oral Pathology Log Book were included in this study regardless of their age, 16 % of them were diagnosed to have fibroma and out of that, 29% of them were male and 79% female. The peak incidence of the lesion was in the 3 rd decade of life. The lesions occurred in the tongue, lip mucosa, sulcus region and buccal mucosa were each to be 12.9%, 12.9% , 32.2 % and 41.9%. This study shows that fibroma is one of the common oral mucosal lesion and it occurred mostly in the 3 rd decade of life where the prevalence is higher in female patients.

The purpose of this study was to evaluate the dental arch relationship of non-syndromic Malay unilateral cleft lip and palate (UCLP) children and also assess the various congenital and postnatal treatment factors that affect dental arch relationship. Study models of 107 UCLP children were included in this study that was treated in Hospital Universiti Sains Malaysia over a period of 12 years (2000-2012). The mean age was 7.69± 2.46 (mean± SD). The dental arch relationship was assessed by GOSLON Yardstick which comprises five categories; named-1: excellent; 2: good; 3: fair; 4: poor; 5: very poor. All the subjects were divided into two groups; favorable (category ratings 1-3) and unfavorable (category ratings 4 and 5) groups. Kappa statistics was used to evaluate the intra-and inter-examiner agreements and logistic regression analysis was used to explore the responsible factors that affect dental arch relationship. The mean GOSLON score was 3.15±0.9. Total 72 subjects (68% of all subjects) were categorized into favourable group (category rating 1, 2 and 3) using GOSLON Yardstick. Intra-and inter-examiner agreements were good to very good. Palatoplasty and cheiloplasty seemed to be correlated with favourable dental arch relationship using crude regression analysis but no significant associations were found. This multivariate study shows no significant association between various congenital and postnatal treatment factors and dental arch relationship.

The challenge arises among researchers when hair follicle stem cells (HFSCs) derived from a human hair follicle remain poorly expanded in defined culture medium. In this study, we isolated the HFSCs and they became confluent after 10 days of cultivation. Comparing the viability of HFSCs cultured in defined keratinocytes serum free medium (KSFM) in a coated plate and CnT07 medium in an uncoated plate, the number of HFSCs cultured in CnT07 was significantly higher at days 2, 4, 6 and 8 (P=0.004). The population doubling time of HFSCs was 21.48±0.44 hours in non-coated plates with CnT07 and 30.73±0.75 hours in coated plates with KSFM. Our primary HFSC cultures were positive for CD200 and K15 with brownish color. Flow cytometry analysis showed that the percentage of HFSCs expressing CD200 and K15 were 65.20±3.16 and 72.07±6.62 respectively. After reaching 100% confluence, the HFSCs were differentiated into an epidermal layer in vitro using CnT02-3D defined media. HFSCs were differentiated into an epidermal layer after 2 weeks of induction. Involucrin- and K6-positive cells were detected in the differentiated epidermal layer. This method is a simple technique for HFSC isolation and has a lower cost of processing and labor, and it represents a promising tool for skin tissue engineering.

Intermaxillary (IMF) screws feature several advantages over other devices used for intermaxillary fixation, but using cone beam computed tomography (CBCT) scans to determine the safe and danger zones to place these devices for all patients can be expensive. This study aimed to determine the optimal interradicular and buccopalatal/buccolingual spaces for IMF screw placement in the maxilla and mandible. The CBCT volumetric data of 193 patients was used to generate transaxial slices between the second molar on the right to the second molar on the left in both arches. The mean interradicular and buccopalatal/buccolingual distances and standard deviation values were obtained at heights of 2, 5, 8 and 11 mm from the alveolar bone crest. An IMF screw with a diameter of 1.0 mm and length of 7 mm can be placed distal to the canines (2 - 11 mm from the alveolar crest) and less than 8 mm between the molars in the maxilla. In the mandible, the safest position is distal to the first premolar (more than 5 mm) and distal to the second premolar (more than 2 mm). There was a significant difference (p<0.05) between the right and left quadrants. The colour coding 3D template showed the safe and danger zones based on the mesiodistal, buccopalatal and buccolingual distances in the maxilla and mandible.The safest sites for IMF screw insertion in the maxilla were between the canines and first premolars and between the first and second molars. In the mandible, the safest sites were between the first and second premolars and between the second premolar and first molar. However, the IMF screw should not exceed 1.0 mm in diameter and 7 mm in length.

Background: Patients presenting with congenitally missing teeth are relatively common and the aim of the dental team is to create a functional, healthy, and aesthetically acceptable dentition. The consequences of missing teeth include an abnormal occlusion or an altered facial appearance which may lead to psychological distress in some patients. Methods: The present case report describes a 23 year old patient with non-syndromic, congenitally missing permanent teeth (CMMPT). Clinical and radiographic examinations revealed agenesis of eight permanent teeth including the third molars. Following interdisciplinary treatment planning, the patient was treated with mechano therapy to correct a class III incisal relationship by space closure in the lower arch. In the upper arch, the right canine was substituted for a missing lateral incisor and space was opened for a prosthetic left lateral incisor (a mini implant). Results: Active orthodontic treatment was completed in 19 months. The management of CMMPT requires an interdisciplinary approach to achieve improved occlusal function and aesthetics. Conclusion: A combined orthodontic-implant-prosthodontic treatment approach can achieve an improved functioning occlusion, and favorable aesthetics.