Ethanol extract of Physalis peruviana and water extract of Hyphaene thebaica were tested for their inhibitory effects on eight bacterial strains (Escherichia coli, Proteus mirabilis, Salmonella typhimurium, Salmonella entrica , Shigella dysenteriae , Bacillus cereus, Bacillus licheniformis and Staphylococcus aureus) by using well diffusion technique. The study demonstrated that Staph. aureus was the most sensitive gram-positive strain and E. coli was the most sensitive gram-negative strain to ethanol extract of Physalis peruvianae and water extract of Hyphaene thebaica. The inhibition zone diameters near to the synthetic antibiotic (ciprofloxacin). The antioxidant activity of the both extracts was also investigated by using 2, 2-diphenyl, 1-picrylhydrazyl (DPPH) scavenging activity and ascorbic acid as control. The results showed that the scavenging effects of both extracts on DPPH radicals increased by increasing the concentration. The ethanolic extract of Physalis peruvianae and water extract of Hyphaene thebaica were a promising source of natural antimicrobial agent, antioxidant agent and as natural preservative of processed food.
Pot experiment was carried out during May 2019 at private farm in el Manyial, Cairo, Egypt. The investigation target was to find out the difference between using plant growth promoting rhizobacteria (B. cereus, Streptomyces chibaensis, B. megaterium, B. polymyxa) either individual or in combination with each other and mineral fertilization on antimicrobial activity and some phytochemical constitutes of Moringa olifera leaves. The highest values of antimicrobial activity recorded for mineral fertilization on treatment followed by mixed biofertilization treatment (GP4) (B. megaterium, B. polymyxa) against seven foodborne pathogens Escherichia coli, Salmonella typhimurium, Staphylococcus aureus, Pseudomonas aeruginosa, Aspergillus niger, Rhizopus stolonifer and Botrytis cinera, as compared with the control treatment. Biofertilization treatment (B. megaterium, B. polymyxa) recorded increasing over control in (GP10) by 160.8% and decreasing of chemical fertilization by 5.41% for polyphenol content, also, the antioxidant content shown increasing over the control (GP10) by 81.1% and decreasing of chemical fertilization (GP9) by 5.6% as well as vitamin C and A were increased with mineral and biofertilizer (GP4) treatments after 55 days. Biofertilization in (GP4) recorded increasing over control group (GP10) by 48.89%, 27.08% and 58% for vitamin A, C and chlorophyll respectively. Ethyl acetate extract was found to be most potent extract than ethanol and water against all tested microorganism.
Twenty eight crude water extracts of four Egyptian plants: Hibiscus sabdariffa (Karkade) , Tamarindus indica (Tamarind) t Glycyrrhiza glabra (Liquorice) and Ceratonia siliqua (Carob) , were tested for their antibacterial activities against 10 common pathogenic bacteria,Bacillus cereus, Shigella flexenary and Escherichia coli showed sensitivity to the 28 extracts. Shigella sp. showed sensitivity to 24 extracts while Pseudomonas sp. and Salmonella typhimurium were sensitive to 23 extracts. Salmonella paratyphi and Aerobacter aerogenes showed sensitivity to 22 extracts, Serratia marcesence and Aeromonas hydrophila showed sensitivity to 20 extracts. Karkade exhibited marked antibacterial activity against all the 10 organisms. E. coli and Shigella flexenary had no observed sensitivity to Liquorice extracts. Aerobacter aerogenes was the only one which had no observed sensitivity to Tamarind extracts. All the extracts of Carob were effective against only two pathogens:Bacillus cereus and Shigella flexenary. The results support the traditional uses of extracts of these plants for the management of bacterial infections and for the development of antibacterial agents for the preservation of foods.
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