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Oocyte cryopreservation is a strategic tool for assisted reproduction, but has limited use due to the complex cellular structure of oocytes, which leads to sub-optimal survival rates. In this study, we used the SPOM in vitro maturation system, which is based on supplementation of cAMP modulators in order to extend meiotic arrest and improve oocyte maturation. cAMP modulators (Forskolin and IBMX) were administered in a short term culture (STC) before or after vitrification, followed by an extended maturation with cilostamide. We hypothesized that a STC with cAMP modulators would improve immature oocyte health and enhance cryotolerance. We found vitrification caused oocyte damage in a great extent, impairing nuclear maturation rates in all vitrified groups (Percentage of matured oocytes: CONT FRESH 77.8 c ; CONT VIT 31.4 ab ; STC/VIT 39.5 b ; VIT/STC 18.6 a). Vitrification also promoted degradation of cytoskeletal actin filaments (Percentage of

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