The present study was included the assessment of the antimicrobial activity of AgNPs synthesized by Punica granatum peel extract against pathogenic bacteria by testing warm aqueous P. granatum peel extract and silver nanoparticles. Punica granatum indicated potency for AgNP extracellular nanobiosynthesis after addition of silver nitrate (AgNO3) 4mM to the extract supernatant, in both concentrations (100mg and 50mg). The biogenic AgNPs showed potency to inhibit both gram-negative and gram-positive bacterial growth. Zons of inhibition in (mm) was lesser in gram-positive than gram-negative bacteria. The resulted phytogenic AgNPs gave higher biological activity than warm aqueous Punica granatum peel extract. The inhibition zone of the phytogenic AgNPs on E. coli reached 17.53, 22.35, and 26.06 mm at (0.1, 0.5, and 1) mg/ml respectively. While inhibition zones of Punica warm aqueous extract reached 5.33, 10.63, and 16.08 mm at the same concentrations. phytogenic AgNPs gave smaller inhibition zones in gram-positive than gram- negative. Cytotoxic activity of the phytogenic AgNPs was assayed in vitro agaist human blood erythrocytes (RBCs), spectroscopic results showed absorbance at 540 nm hemolysis was observed. In general, AgNPs showed least RBCs hemolysis percentage, at 1 mg/ml concentration, hemolysis percentage was (4.50%). This study, concluded that the Punica granatum peel extract has the power of synthses of AgNPs characterized by broad spectrum antimicrobial activity with cyto-toxicity proportional to AgNPs concentration.
The callus induction by use 1.5mg/l of α-naphthalene acetic acid (NAA) as auxin and 2mg/l of BA as cytokinins were added to MS medium that prepared as above before sterilize step. Sodium Chloride and Lysine effect on callus induction and growth of Fenugreek (Trigonella foenum-graecum L.).Five concentration of Sodium Chloride Salts (0, 2, 4, 6 and 8) were added to the culture medium and five concentrations of lysine (0, 10, 20, 30and 40mg/l) added to cultured medium. Results showed that NaCl at (2g/l) increased callus induction, fresh weight, dry weight and potassium ions of callus. The study also showed that (2 g \l) of NaCl was signification increased Sodium and Chloride ions of callus. Lysine (Lys) effect on callus characteristics:-The treatment 40mg\l outperformed of the rest treatments including control by gave the highest values of callus fresh and dry weights, as well as ions, soluble carbohydrates
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