The callus induction by use 1.5mg/l of α-naphthalene acetic acid (NAA) as auxin and 2mg/l of BA as cytokinins were added to MS medium that prepared as above before sterilize step. Sodium Chloride and Lysine effect on callus induction and growth of Fenugreek (Trigonella foenum-graecum L.).Five concentration of Sodium Chloride Salts (0, 2, 4, 6 and 8) were added to the culture medium and five concentrations of lysine (0, 10, 20, 30and 40mg/l) added to cultured medium. Results showed that NaCl at (2g/l) increased callus induction, fresh weight, dry weight and potassium ions of callus. The study also showed that (2 g \l) of NaCl was signification increased Sodium and Chloride ions of callus. Lysine (Lys) effect on callus characteristics:-The treatment 40mg\l outperformed of the rest treatments including control by gave the highest values of callus fresh and dry weights, as well as ions, soluble carbohydrates