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Reactive oxygen species (ROS)‐induced mitophagy is associated with a variety of diseases. Therefore, visualizing and modulating the process of ROS‐induced mitophagy is essential for understanding the role of mitophagy in cellular homeostasis, physiological processes, and pathogenesis. Herein, using fluorescence lifetime imaging microscopy (FLIM), for the first time the complete dynamic process of PINK1/Parkin pathway‐mediated mitophagy is described. Induced by the photo‐controlled release of ROS, nitrogen‐doped multi‐functional carbon nanozymes (ENZ‐NCDs) is used as a probe to visualize and quantitatively study ROS‐induced mitophagy. The successful preparation of ENZ‐NCDs provides a potentially powerful tool and a new strategy for real‐time mitophagy monitoring and future quantification of mitochondrial damage caused by ROS and ROS‐induced mitophagy‐related diseases.

The use of carbon dots (CDs) with dual emission based on ratiometric fluorescence has been attracting attention in recent times for more accurate ion detection since they help avoid interference from background noise, probe concentration, and complexity. Herein, novel dual-emission nitrogen-doped CDs (NCDs) were prepared by a simple method for Cu2+ and ClO- detection. The NCDs showed excellent anti-interference ability and selectivity for different emissions. In addition, a good linear relationship was observed between the fluorescence intensity (FI) of the NCD solutions in different emissions with Cu2+ (0–90 μM) and ClO- (0–75 μM). The limits of both Cu2+ detection and ClO- were very low, at 17.7 and 11.6 nM, respectively. The NCDs developed herein also showed a good recovery rate in water for Cu2+ and ClO− detection. Hence, they are expected to have a more extensive application prospect in real samples.

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