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A sensitive fluorimetric method for the determination of epinephrine (E) is described in this paper. The experiments indicate that epinephrine can react with formaldehyde (HCHO) in an acid medium to form a condensation product, which can be oxidized by potassium hexacyanoferrate(III) (K3[Fe(CN)6]) in borax buffer (pH = 9.5). The reaction product can emit strong fluorescence. Ascorbic acid (AA) is used in order to consume excess potassium hexacyanoferrate and stabilize the fluorescent product. Under optimum conditions, a linear relationship has been obtained between the fluorescence intensity and the concentration of epinephrine in the range of 1.4 x 10(-9) - 2.1 x 10(-6) mol/l, and the detection limit is 2.4 x 10(-10) mol/l (4.3 x 10(-11) g/ml, S/N = 3). The method is applied for the determination of E in both actual sample and the synthetic sample with E and norepinephrine (NE) by using the coupling technique of synchronous fluorimetry and H-point standard addition method, and the results obtained are satisfactory.

This article describes a new method for analysis of IR fingerprint spectra (FPS), which characterizes traditional Chinese medicine (TCM). Two new indices of IR FPS are introduced, namely, common peak ratio and variation peak ratio. Their values are calculated by means of the sequent analysis method, in which each sample's IR FPS of the components extracted from Glycyrrhizae root is as a standard, and the sequences are made in accordance with the values of their

SummaryThe enantiomers of eight O,O-dialkyl-2-benzyloxycarbonyl-aminoarylmethyl-phosphonates are directly separated on an N-(3,5-dinitrobenzoyl)leucine (DNBleu) column. The influence of the mobile phase composition and the column temperature on the retention and the enantioselectivily are investigated. The influence of the length and steric hindrance of alkoxyl groups of the phosphonate ester and the nature of the substituent p-CI and pH on the benzene ring which is attached to the chiral carbon atom on chiral separation are discussed also.

The synthesized DPTP polymer was statically coated on the capillary column to prepare the DPTP column for separating multiple analytes.