Summary Boysenberry, a hybrid Rubus berry, is mainly cultivated in New Zealand. We previously reported that consumption of boysenberry juice (BBJ) exhibited anti-obesity effects in high-fat feeding rats. In this study, we focused on the suppressive effect of BBJ and its fraction on triglyceride absorption from the gastrointestinal tract. BBJ effectively inhibited pancreatic lipase activity in vitro, and was separated into four fractions (Fr1, Fr2, Fr3 and Fr4) by HP-20 column chromatography. Among all the fractions, Fr3, the ellagic acid-rich fraction, showed the most potent inhibition against pancreatic lipase in vitro with Fr2, the anthocyanin-rich fraction, second. Authentic ellagic acid equivalent in Fr3 showed poor activity against pancreatic lipase. Then, each fraction was orally administered with corn oil to rats fitted with a jugular catheter to examine the effects of each fraction on plasma triglyceride levels. Both Fr2 and Fr3 effectively suppressed the plasma triglyceride level elevation at a dose of 1,000 mg/kg body weight. These findings demonstrated that BBJ contains chemical components which inhibit triglyceride absorption from the gastrointestinal tract.
Recently, it has often been reported that certain polyphenols are useful for prevention of obesity. However, few investigations have been conducted on the anti-obesity effects of boysenberry polyphenols, including anthocyanins and ellagic acid. Therefore, we examined whether boysenberry juice BJ would prevent obesity in Wistar rats fed normal and high-fat diets containing 5 BJ for 12 weeks. At the end of the feeding period, our data revealed significant decreases in body weight gain, body fat accumulation, and liver lipid levels, especially triglyceride TG and total cholesterol, in the rats fed diets containing 5 BJ BJ group compared with the rats fed diets without BJ control group. Furthermore, we measured the plasma TG levels in rats fed each test diet after a 20-hour fast, and observed notable reductions in the postprandial plasma TG levels in the BJ group compared with the control group after 3 hours. These results suggest that consumption of BJ has potential preventive effects against obesity induced by a high-fat diet and associated metabolic syndrome.
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