Endo-β-1, 4-xylanases is an enzyme that depolymerize xylan, a major component of lignocelluloses. Lignoselulose is a great source of cheap carbohydrate and thus has been used over the past decade as a raw material for the production of high value products, such as enzymes. However, lignocelluloses are a highly recalcitrant material that is extremely difficult to depolymerize. By using proper pretreatment, lignocelluloses from agroindustrial biomass can replace conventional carbon sources in media preparation for enzyme production. In this study, we used three kinds of agroindustrial biomass, such as sorghum variety of Buleleng, sorghum variety of JP, rice straw LIPI GO1 and these biomasses were pretreated with acid to remove a portion of lignin for use as a carbon source to endoxylanase production. The production of xylanase Kitasatospora sp. under submerged fermentation was investigated with different carbon sources using agroindustrial biomass. Optimization steps included studies carbon source concentration and pH medium fermentation. The optimized condition of enzyme production was obtained using the sorghum variety Buleleng biomass at 2% concentration and pH medium is 9.0 with activity 3. 04 U/mL.

This study aims to make adhesives with a variety of concentration extenders. First, the coconut husk was delignified with 25% NaOH and then precipitated with sulfuric acid. The yield of lignin from coconut coir was 38.92%, with a purity of 68%, a phenolic hydroxyl content of 2.47%, and an equivalent molecular weight of 1666.67 g/mol. Lignin was then used to synthesize phenol-formaldehyde (LPF) lignin by adding tapioca flour extender with variations of 10%, 20% and 30% of the mass of phenol through the resol method with 50% NaOH catalyst. Then the characterization was carried out using FTIR Spectrophotometer, XRD and adhesive test. The results showed that the LPF copolymer with a 10% extender was better than the LPF copolymer without an extender for wood adhesives with adhesive resistance of 955.34 N and low formaldehyde emission of 0.44 mg/L.