Experimental work in wood saccharification requires methods for the anelysls of reducing suger, fermentable sugars, end alcohol that are dependable, simple, rapid, and capable of being run in large numbers. Two methods were used for the determination of reducing sugars-one an electrometric titration method and the other a micromethod of Shaffer and Somogyi. The electrometric titration method was used when it was desirable to have a sugar analysis in as short a time as possible; the micromethod when large numbers of IN STUDIES of wood saccharification it is necessary to have available analytical methods for the determination of the sugars produced, the amount of fermentable sugars, and the amount of alcohol produced when the wood sugars are fermented. Because wood sugars consist of mixtures of several sugars as well as dissolved lignin, furfural, formic and acetic acid, various oils, and other products in the wood, their analysis presents special problems. To be of most use in following the work, the analysis should be simple, rapid, and capable of being run in large numbers.
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