The neuropeptide arginine vasopressin (AVP) plays significant roles in maintaining homeostasis and regulating social behavior. In vaginally delivered neonates, a surge of AVP is released into the bloodstream at levels exceeding release during life-threatening conditions such as hemorrhagic shock. It is currently unknown where the potential sites of action are in the neonate for these robust levels of circulating AVP at birth. The purpose of this study is to identify the location of AVP receptor 1a (AVPR1A) sites as potential peripheral targets of AVP in the neonatal mouse. RT-qPCR analysis of a sampling of tissues from the head demonstrated the presence of Avpr1a mRNA, suggesting local peripheral translation. Using competitive autoradiography in wildtype (WT) and AVPR1A knockout (KO) postnatal day 0 (P0) male and female mice on a C57BL/6J background, specific AVPR1A ligand binding was observed in the neonatal mouse periphery in sensory tissues of the head (eyes, ears, various oronasal regions), bone, spinal cord, adrenal cortex, and the uro-anogenital region in the neonatal AVPR1A WT mouse, as it was significantly reduced or absent in the control samples (AVPR1A KO and competition). AVPR1A throughout the neonatal periphery suggest roles for AVP in modulating peripheral physiology and development of the neonate. Materials and methods Animals. Avpr1a mice 18 , fully backcrossed to C57BL/6J 19 , were bred in house. All breeding and experimental protocols were performed with the approval of Institutional Animal Care and Use Committee of Florida State University (protocols 1722 and 1746) in accordance with state and federal guidelines (Guide for the Care and Use of Laboratory Animals of the National Institutes of Health). Breeder pairs of heterozygous Avpr1a mice were housed in a temperature and humidity-controlled environment, on a 12 h light/dark cycle with food and

Prediction equations have been considered an accurate method for estimating resting metabolic rate (RMR) across multiple populations, but their accuracy for college-aged individuals not on an athletics team remains to be determined. Sixty-two college-aged (18 - 30 yrs.) males (n = 31) and females (n = 31) had their RMR measured (RMRm), using indirect calorimetry, and body composition assessed via air-displacement plethysmography. The World Health Organization (WHO), Mifflin-St Jeor (Mifflin), Harris-Benedict (HB), Cunningham, and Nelson equations were used to estimate RMR. No difference was observed between the Cunningham and RMRm regardless of sex (p≥0.05). All other prediction equations estimated a significantly lower RMR for males (p<0.05). The Mifflin and Nelson equations predicted an RMR that was significantly lower than RMRm for females (p<0.05). When compared to RMRm, no difference was detected for females using the WHO, HB, or Cunningham (p≥0.05). Only the Nelson equation predicted an RMR that was outside of the clinically acceptable range (±10% of RMRm) regardless of sex. The Cunningham, WHO, and HB equations can accurately predict RMR for college-aged males and females. RMR prediction equations used in this study are less accurate for those with greater RMRs. Key Findings: • For adults 18-30 years old that are not on an athletics team, the Cunningham equation can accurately predict RMR. • The Nelson equation should not be used to predict RMR for this population. • There is a systemic bias for RMR prediction equations to underestimate higher measured RMR values.