An artificial DNA duplex, each strand consisting of 45 monomers, is constructed from chemically synthesized deoxyriboöligonucleotides. The resulting bihelical polymer may code for a modified S-peptide of Ribonuclease A. This is the first synthetic duplex designed to code for a eukaryotic message.

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Large amounts of a heat-inducible phage lambda mutant (XCI857) may be obtained under standardized conditions. The phage is harvested by simple polyethylene glycol (C 6000) precipitation and purified by CsCl density gradient banding. Deoxyribonucleic acid (DNA) is extracted by cold phenol and purified by sucrose density gradient sedimentation to yield a homogenous population of unbroken X DNA molecules.

Large amounts of a heat-inducible phage lambda mutant (λCI857) may be obtained under standardized conditions. The phage is harvested by simple polyethylene glycol (C 6000) precipitation and purified by CsCl density gradient banding. Deoxyribonucleic acid (DNA) is extracted by cold phenol and purified by sucrose density gradient sedimentation to yield a homogenous population of unbroken λ DNA molecules.