There has been no report on the prevalence of
Campylobacter
spp. in farm animals in Mongolia. To uncover the prevalence of
Campylobacter
spp. in chickens in Mongolia and their antimicrobial resistance, in this study, we isolated and characterized
Campylobacter
spp. from chickens in Mongolia. We collected 71 cloacal swabs of chickens from 5 farms including 4 layer farms and one broiler farm near Ulaanbaatar city and isolated 25
Campylobacter jejuni
and 6
Campylobacter coli
isolates. All isolates were resistant to tetracycline, and 3
C. coli
isolates were resistant to erythromycin. The
C. coli
isolates possessed either the
erm
(B) gene or nucleotide substitution at nt 2,075 of 23S rDNA, both of which are known to be associated with erythromycin resistance. Sixteen of the 31
C. jejuni/C. coli
isolates (51.6%) were resistant to nalidixic acid and fluoroquinolones. All the fluoroquinolone-resistant isolates possessed amino acid substitution from threonine to isoleucine at codon 86 (nucleotide substitution: A
C
A to A
T
A). Multilocus sequence typing and phylogenetic analyses showed a variation in
C. jejuni
/
C. coli
in chickens in Mongolia. In addition, some of the
C. jejuni
isolates seemed to be phylogenetically close to isolates in Asian and Oceanian countries. This is the first report on the characterization of antimicrobial resistance of
Campylobacter
spp. in farm animals in Mongolia and is valuable for implementation of measures for a prudent use of antimicrobials in farm animals.
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