Infrared spectroscopy is often used as a simple, fast, and green method to screen for economically motivated adulteration in spices. However, conventional microscopy remains the reference method. In this research, the combination of microscopy and Fourier-transform infrared spectroscopy in mapping mode, namely, micro-FTIR imaging, along with Principle Component Analysis were used to develop a non-targeted method for detecting a broad range of organic and mineral bulking agents that could potentially be used to adulterate black pepper. This method, based on the spatial distribution of black pepper chemical composition, has been thoroughly validated as a one-class, non-targeted classification method. Results are categorized as Typical or Atypical, where an Atypical result indicates a high probability of adulteration. For an Atypical outcome, a multitool investigational approach is then used for the detection and identification of the potential adulterant.

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