Bacteriocins are antibacterial proteins created by bacteria and its effective against other strains of bacteria which are closely linked to the producing strains and a number of species from the same family. The aim of current study was to evaluate the activity of bacteriocin that extracted from clinical isolates of Klebsiella pneumonia against different species of enterobacteriaceae in both planktonic and biofilm state. The antibacterial activity of bacteriocins (klebocins) from Klebsiella pneumoniae isolates towards diverse pathogenic species from enterobacteriaceae (by well assay method) was demonstrated , however the antibiofilm activity against some species of enterobacteriaceae was studied also by Tissue culture plate method (TCP) in first and last stages of biofilm formation .The results revealed that klebocin with different concentrations was efficient against different pathogenic species by producing different inhibition zones. Results also showed that klebocins had a wide antibiofilm activity on some pathogenic species of Gram-negative bacteria .In addition to these finding it was observed that the antibiofilm activity of klebocin on premature biofilm of Klebsiella was higher than its effect towards other pathogenic species, while it was affected on mature biofilm of other bacterial species as well as Klebsiella.

The Indian costus plasma properties are investigated including electron temperature (Te), "electron density (ne)", "plasma frequency (fp)", " Debye sphere length", and amount of Debye(Nd),  using the spectrum of optical emission technique. There are several energies used, with ranging from 300 to 600 mJ. The Boltzmann Plot is used to calculate the temperature; where as Stark's Line Broadening is used to calculate the electron density. The Indian costus was spectroscopically examined in the air with the laser  at 10 cm away from the target and the optical fiber  at 0.5 cm away. The results were obtained for an electron temperature range of (1.8-2.2) electron volts (ev) and a wavelength range of (300-600) nm. The XRF analysis reveals that the Indian costus contains a variety of minerals, each with a different percentage, which explains why the optical emission spectrum has so many peaks. When the laser energy is  (between 300 and 600 mJ) the  "optical emission spectroscopy (OES) "has been  used to analyze the plasma spectrum of the Indian costus in the air. The results shows that as the laser energy grew, the amount of Debye will be greater i-e (Nd) >>>1,  which is one of the plasma properties.