Two hundred swab samples were collected from burn patients and burn units for the period, beginningfrom August 2012 to the end of April 2013 from a number of hospitals in Baghdad governorateincluding: Al-Kindi General Teaching Hospital, Al-Yarmuk General Teaching Hospital and Al-Imam AliHospital. The collected samples were cultured on different media and tested biochemically in order to findout the profile of bacteria that colonize burn patients wounds and environment of burn units. The resultsof bacterial culturing revealed that out of 200 samples, 105 samples 52.5% were observed to havebacterial growth (positive samples), while negative samples represented 95(47.5%). The profile of thebacteria in the cultured samples revealed: Pseudomonas aeruginosa 40.95% was the most common isolatefollowed by Staphylococcus aureus 20.0%, Klebsiella pneumoniae 17.1%, Escherichia coli 8.5%,Pseudomonas putida 4.76%, Enterobacter aerogenes 3.80%, Acinetobacter baumannii 2.85% andProteus mirabilis 1.90%. Forty three P. aeruginosa isolates were tested for antibiotic susceptibility. Theresults showed most isolates were potentially resistant to different antibiotics as follow, all isolates 100%had resistance to Ceftriaxone, Cefepime, and Chloramphenicol, and showed high resistance toTobramycin 95.3%, Gentamicin 93.0%, Ceftazidime 88.3%, Cefotaxime 86.0%, Piperacillin 83.7% andAmikacin 79.0%, beside illustrating low resistance to Aztreonam 67.4%, Ciprofloxacin 46.5%, andImipenem 13.9% among these antibiotics, Imipenem was the most effective antibiotic because 86.0% ofthe isolates appeared to be high sensitive to it.