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Bactericidal efficacy of gold nanoparticles conjugated with ampicillin, streptomycin and kanamycin were evaluated. Gold nanoparticles (Gnps) were conjugated with the antibiotics during the synthesis of nanoparticles utilizing the combined reducing property of antibiotics and sodium borohydride. The conjugation of nanoparticles was confirmed by dynamic light scattering (DLS) and electron microscopic (EM) studies. Such Gnps conjugated antibiotics showed greater bactericidal activity in standard agar well diffusion assay. The minimal inhibitory concentration (MIC) values of all the three antibiotics along with their Gnps conjugated forms were determined in three bacterial strains,Escherichia coli DH5α,Micrococcus luteusandStaphylococcus aureus. Among them, streptomycin and kanamycin showed significant reduction in MIC values in their Gnps conjugated form whereas; Gnps conjugated ampicillin showed slight decrement in the MIC value compared to its free form. On the other hand, all of them showed more heat stability in their Gnps conjugated forms. Thus, our findings indicated that Gnps conjugated antibiotics are more efficient and might have significant therapeutic implications.

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This paper presents a forecasting model designed using WSNs (Wireless Sensor Networks) to predict flood in rivers using simple and fast calculations to provide real-time results and save the lives of people who may be affected by the flood. Our prediction model uses multiple variable robust linear regression which is easy to understand and simple and cost effective in implementation, is speed efficient, but has low resource utilization and yet provides real time predictions with reliable accuracy, thus having features which are desirable in any real world algorithm. Our prediction model is independent of the number of parameters, i.e. any number of parameters may be added or removed based on the on-site requirements. When the water level rises, we represent it using a polynomial whose nature is used to determine if the water level may exceed the flood line in the near future. We compare our work with a contemporary algorithm to demonstrate our improvements over it. Then we present our simulation results for the predicted water level compared to the actual water level.Comment: 16 pages, 4 figures, published in International Journal Of Ad-Hoc, Sensor And Ubiquitous Computing, February 2012; V. seal et al, 'A Simple Flood Forecasting Scheme Using Wireless Sensor Networks', IJASUC, Feb.201