Mikroorganisme alami yang ada dalam tubuh manusia disebut mikroorganisme normal atau flora normal, namun dalam keadaan tertentu dapat bersifat patogen dan menimbulkan penyakit infeksi salah satunya Staphylococcus Aureus. Bakteri ini sering resisten terhadap berbagai jenis obat sehingga mempersulit pemilihan antimikroba yang sesuai untuk terapi. Bawang putih (Allium Sativum) mempunyai bahan aktif yaitu alicin, flavonoid, ajoene, minyak atsiri dan saponin yang mempunyai efek antimikroba. Tujuan penelitian ini adalah mengetahui daya hambat ekstrak bawang putih (Allium Sativum) terhadap pertumbuhan bakteri Staphylococcus aureus. Penelitian ini merupakan jenis penelitian True Experiment dengan rancangan Posttest dengan Kelompok Kontrol (Posttest Only Control Group Design), menggunakan Rancangan Acak Lengkap (RAL) dengan 24 perlakuan dan 4 kali ulangan. Sampel yang digunakan dalam penelitian ini adalah bakteri Staphylococcus aureus yang diberi perlakuan ekstrak bawang putih (Allium Sativum) dengan konsentrasi 250 µg/mL, 500 µg/mL, 750 µg/mL, 1000 µg/mL, 2 kelompok kontrol terdiri dari kontrol positif ciprofloksasin dan kontrol negatif aquades steril. Daya hambat diperoleh berdasarkan pengukuran zona hambat dan data yang diperoleh dari analisis menggunakan uji One Way Anova. Hasil pengukuran zona hambatan bakteri Staphylococcus aureus pada masing-masing konsentrasi berturut turut adalah, konsentrasi 250 µg/mL sebesar 6.5 mm; 500 µg/mL,13.3 mm; 750 µg/mL, 15 mm dan 1000 µg/mL, 17.8 mm. Pada konsentrasi yang paling tinggi menghasilkan daerah zona hambat yang terlebar. Hasil uji One Way Anova diperoleh nilai signifikan yakni 0,008 lebih kecil dari p value: 0,05 sehingga dapat dinyatakan bahwa konsentrasi ekstrak bawang putih (Allium Sativum) dapat mempengaruhi pertumbuhan bakteri Staphylococcus Aureus.
Diarrhea is an endemic disease that causes individuals to excrete feces with a liquid consistency that have frequency of three or more times a day. The disease is caused by gastrointestinal infection due to the introduction of disease agents that can be transmitted through food, water, eating utensils, hands and flies. One of the causative agents of diarrhea is Escherichia coli. The use of various types of diarrhea drugs is basically to prevent the proliferation of Escherichia coli bacteria in the gastrointestinal tract, such as the use of Kawista fruit pulp and seeds by local people as a cure for diarrhea. The purpose of this study was to test the inhibitory activity of methanol extract of Kawista fruit pulp and seeds (Limonia acidissima L. ) Dompu variant with 4 concentrations, 25%, 50%, 75% and 100% extract concentrations. Testing antibacterial activity using the agar well diffusion method, which is done by measuring the inhibition zone around the wells. The methanol extract of Kawista fruit pulp and seeds (Limonia acidissima L. ) Dompu variant was significantly able to inhibit the growth of Escherichia coli at extract concentrations of 25%, 50%, 75% and 100% with inhibition zone diameter respectively 14.5 mm, 20.5 mm, 25.25 mm and 26.75 mm. The 100% extract concentration is classified as the concentration with the highest effectiveness in inhibiting the growth of Escherichia coli bacteria. Based on the results obtained, it can be concluded that the methanol extract of Kawista fruit pulp and seeds (Limonia acidissima L)
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