Diarrhea is an endemic disease that causes individuals to excrete feces with a liquid consistency that have frequency of three or more times a day. The disease is caused by gastrointestinal infection due to the introduction of disease agents that can be transmitted through food, water, eating utensils, hands and flies. One of the causative agents of diarrhea is Escherichia coli. The use of various types of diarrhea drugs is basically to prevent the proliferation of Escherichia coli bacteria in the gastrointestinal tract, such as the use of Kawista fruit pulp and seeds by local people as a cure for diarrhea. The purpose of this study was to test the inhibitory activity of methanol extract of Kawista fruit pulp and seeds (Limonia acidissima L. ) Dompu variant with 4 concentrations, 25%, 50%, 75% and 100% extract concentrations. Testing antibacterial activity using the agar well diffusion method, which is done by measuring the inhibition zone around the wells. The methanol extract of Kawista fruit pulp and seeds (Limonia acidissima L. ) Dompu variant was significantly able to inhibit the growth of Escherichia coli at extract concentrations of 25%, 50%, 75% and 100% with inhibition zone diameter respectively 14.5 mm, 20.5 mm, 25.25 mm and 26.75 mm. The 100% extract concentration is classified as the concentration with the highest effectiveness in inhibiting the growth of Escherichia coli bacteria. Based on the results obtained, it can be concluded that the methanol extract of Kawista fruit pulp and seeds (Limonia acidissima L)

Powdery mildew is one of melon disease caused by ascomycetes fungus from genus Sphaerotheca and Erysiphe. Breeding is considered to be the best approach to control Powdery mildew infection especially in melon. MG3 is a melon cultivar derived from ‘MG1’ x ‘Ladika’ and its estimate carry out resistant gene to Powdery mildew infection inherited from MG1. This research is to find out percentage and phenotype characters of Powdery mildew infection in MG3 compared to four commercial melons (Action 434, Glamour, MAI and ladika) that are popular in the market. Five melons are planted in greenhouse B of KP4 UGM. After five weeks, the plants where inoculated with Powdery mildew KP4 UGM in leaves. Scoring in three days after inoculation and the percentage of infections measured every 3 days for eight times using grid line 21 cm x 21 cm with small cubics 1,5 cm x 1,5 cm. The analysis of infections level in leaves, plant and population was done using Fukino protocol. The results show that cultivar MG3 tolerant and stable to Powdery mildew infection compare to cultivar Action 434, MAI and ladika in leaves, plant and population level. The infection of Powdery mildew caused damage to the leaves tissue, decreasing the abilities of photosynthesis resulting in necrotic, chlorotic and dead of the infected leaves. In fruits, infection causes break in the net, immature and rotten flesh, decrease sweetness and scent in cultivar Action434, MAI and Ladika. Base on microscopic analysis, Powdery mildew isolat KP4 UGM is a member of from genus Podosphaera.

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